中国畜牧兽医2017，Vol.44Issue(2)：613-618,6.DOI:10.16431/j.cnki.1671-7236.2017.02.043
养殖锦鲤鲤鱼浮肿病的检测与鉴定
Testing and Identification of Carp Edema Virus Disease in Cultured Koi
摘要
Abstract
An acute infectious diseases occurred in a koi farm in Fangshan district,Beijing,and it resulted in mortalities of more than 50％.The main symptoms of sick koi were gills necrosis,kidney erosion and edema,which were similar to the clinical signs of koi herpes virus disease (KHVD).But PCR tests showed negative results for KHV.For further diagnosis,bacterial cultures,transmission electron microscopy studies,virus isolation and PCR tests were used.Electron microscopic observation revealed pox virus particles having a size of about 200 nm× 400 nm in the kidney.548 and 180 bp fragments were amplified from organs of sick koi by PCR method using specific primers of carp edema virus (CEV).The fragments were sequenced and analysed.The results showed that they were shared 100％ nucleotide identity with CEV-H504.All the results indicated that this disease was carp edema virus disease,caused by a kind of pox virus,CEV.This was the first report on the CEV of cultured koi in China.关键词
鲤鱼浮肿病毒/鲤鱼浮肿病/锦鲤疱疹病毒/锦鲤Key words
carp edema virus/carp edema virus disease/koi herpes virus/koi分类
农业科技引用本文复制引用
徐立蒲,那立海,江育林,王小亮,张文,潘勇,景宏丽,王静波,王姝,曹欢,王澎..养殖锦鲤鲤鱼浮肿病的检测与鉴定[J].中国畜牧兽医,2017,44(2):613-618,6.基金项目
农业部动物疫情监测与防治项目 （）
北京市农业试验示范项目(20160117) （20160117）
现代农业产业技术体系北京市观赏鱼创新团队(GSY-02-09) （GSY-02-09）
