中国循证儿科杂志2016，Vol.11Issue(3)：228-231,4.DOI:10.3969/j.issn.1673-5501.2016.03.014
新生儿金黄色葡萄球菌皮肤和软组织化脓性感染临床分离株分子及毒力特征
Molecular and virulence characterization of methicilin-resistant Staphylococcus aureus strains isolated from neonates with skin and soft tissue infection
摘要
Abstract
Objective To investigate the molecular and virulence characterization of Staphylococcus aureus (SA) isolated from neonates with skin and soft tissue infections(SSTIs) through one-year prospective surveillance study,and to provide the scientific evidence for prevention and control of SA infection.Methods Cases of SSTIs in neonates were prospectively collected from May 2015 to April 2016 in NICU of Beijing Children's Hospital,the clinical data were recorded,the multilocus sequence typing(MLST) and Staphylococcal cassette chromosome mec (SCCmec) types were analyzed by multiplex polymerase chain reaction method,the spa type was analyzed by PCR.PCR was also used to test 23 virulence genes,including 21 superantigen genes,PVL gene and sasX gene.Results A total of 13 SA strains were collected,including 7 MRSA,the most common clone of MRSA was agr Ⅰ-ST59-SCCmec Ⅳ-t437.It was found that all strains contained at least two superantigen toxin genes,and 6 strains were PVL positive.SasX was not detected in any of the isolates.Conclusion The carrying rate of SA from SSTIs in neonates was low,the most common clone of MRSA was agr Ⅰ-ST59-SCCmecⅣ-t437,the carrying rate of superantigen was high.关键词
耐甲氧西林金黄色葡萄球菌/皮肤和软组织化脓感染/毒力/新生儿Key words
Methicillin-resistant Staphylococcus aureus/Skin and soft tissue infections/Virulence/Neonate引用本文复制引用
耿文静,李文婷,姚开虎,沈叙庄,齐宇洁..新生儿金黄色葡萄球菌皮肤和软组织化脓性感染临床分离株分子及毒力特征[J].中国循证儿科杂志,2016,11(3):228-231,4.基金项目
国家自然科学基金资助项目:81171648 （）
北京市优秀人才青年骨干个人资助项目:2014000021469g243 （）
