中国药理学与毒理学杂志2017，Vol.31Issue(4)：325-331,7.DOI:10.3867/j.issn.1000-3002.2017.04.005
麦冬皂苷D′对大鼠心肌细胞H9c2的细胞毒性
Cytotoxicity of ophiopogonin D′ for rat H9c2 cardiomyocytes
摘要
Abstract
OBJECTIVE To study the cardiotoxicity of ophiopogonin D′(OPD′) for rat H9c2 cardio? myocytes. METHODS H9c2 cells were exposed to OPD′ 0.1, 1, 5, 10, 20, 25 and 50 μmol·L-1 for 24 h. Cell viability was examined by MTS assay, and the morphological changes in H9c2 cells were quanti? fied. The cell nucleus injury was examined by high content immune fluorescence screening and the morphological changes were observed under a fluorescence microscope. After treatment with OPD′ 0.1, 1, 5 and 10 μmol·L- 1 for 24 h, the effect on reactive oxygen species (ROS), mitochondrial mem? brane potential(MMP) and apoptosis was detected by flow cytometry. RESULTS The viability was sig? nificantly reduced following exposure to OPD′ 0.1, 1, 5, 10, 20, 25 and 50 μmol·L- 1 (P<0.05,P<0.01). The IC50 value was 9.9 μmol ·L- 1 and cell shrinkage and apoptosis occurred. The levels of ROS and apoptosis rate of H9c2 cells were significantly increased after exposure to OPD′ 0.1, 1, 5 and 10 μmol·L-1 for 24 h (P<0.05,P<0.01) and MMP markedly declined (P<0.05,P<0.01). CONCLUSION OPD′ has significent cytotoxicity on H9c2 cells. It may be related to inducing apopotsis pathways.关键词
麦冬皂苷D′/H9c2细胞/毒性作用Key words
ophiopogonin D′/H9c2 cells/toxic actions分类
医药卫生引用本文复制引用
任思嘉,王宇光,高月,徐焕华,李明,郝斐然,马增春,汤响林,梁乾德,谭洪玲,肖成荣..麦冬皂苷D′对大鼠心肌细胞H9c2的细胞毒性[J].中国药理学与毒理学杂志,2017,31(4):325-331,7.基金项目
国家自然科学基金(81630102) （81630102）
国家自然科学基金(81673633) （81673633）
国家重点基础研究发展计划("973")资助项目(2012CB518402)The project supported by National Natural Science Foundation of China(81630102) （"973"）
National Natural Science Foundation of China(81673633) （81673633）
and National Key Basic Research and Development Program ("973") (2012CB518402) （"973"）
