中国药理学与毒理学杂志2017，Vol.31Issue(4)：367-374,8.DOI:10.3867/j.issn.1000-3002.2017.04.011
钙调磷酸酶抑制剂筛选方法的研究进展
Resevrch progress in screening methods of calcineurin inhibitors
徐翔 1崔志峰 1丁东栋 1朱廷恒1
作者信息
- 1. 浙江工业大学生物工程学院,浙江杭州 310014
- 折叠
摘要
Abstract
Calcineurin inhibitor screening should benefit from the strengths of each method. This paper reviews three calcineurin inhibitor screening methods:yeast "positive screening" model, yeast reporter gene high through- put screening model and calcineurin activity detection method. We have compared the range of application, advantages and disadvantages of each method, and combined effect of multiple methods. The yeast "positive screening" model can prevent the false positiveness caused by bacteriostat by using growth zooms instead of inhibition zooms. The yeast reporter gene high through-put screening model is suitable for pilot screening among a wide range of samples and is more efficient than the other methods. The calcineurin activity detection method can directly report the inhibitory ratio to a specific target. This review is expected provide a model-selection reference for the development of novel low-toxic calcineurin inhibitors.关键词
钙调磷酸酶/抑制剂/筛选方法Key words
calcineurin/inhibitor/screening methods分类
医药卫生引用本文复制引用
徐翔,崔志峰,丁东栋,朱廷恒..钙调磷酸酶抑制剂筛选方法的研究进展[J].中国药理学与毒理学杂志,2017,31(4):367-374,8.
