中成药2017，Vol.39Issue(4)：684-689,6.DOI:10.3969/j.issn.1001-1528.2017.04.004
通络醒脑泡腾片经Nampt/SIRT1/FOXO3途径改善SAMP8小鼠的学习记忆
Tongluo Xingnao Effervescent Tablets ameliorates cognitive function of SAMP8 mice via Nampt/SIRT1/FOXO3 pathway
摘要
Abstract
AIM To investigate the effects of Tongluo Xingnao Effervescent Tablets (Chuanxiong Rhizoma,Angelicae sinensis Radix and Scutellariae Radix) on the learning and memory function in SAMP8 mice,and improvements in cognitive function.METHODS Morris water maze was used to evaluate the change of cognitive function in SAMP8 mice after they were treated with Tongluo Xingnao Effervescent Tablets for 60 d,and the activity of superoxide dismutase (SOD),the concentration of protein carbonyls (PC),the ratio of NAD +/NADH in brain tissue were detected by ELISA,the expression of Nampt,SIRT1 and FOXO3 in hippocampus were measured with immunohistochemical methods.RESULTS Tongluo Xingnao Effervescent Tablets shortened escape latency and obviously increased percentage of time in target quadrant in hidden platform test and increased the times entering the target quadrant in spatial probe test in SAMP8 mice.It evidently enhanced the activity of SOD,the ratio of NAD +/NADH and distinctly decreased the protein carbonyls level.Moreover,Tongluo Xingnao Effervescent Tablets could markedly up-regulate the expression of Nampt and SIRT1,but evidently down-regulate FOXO3 protein expression.CONCLUSION The experimental data show that Tongluo Xingnao Effervescent Tablets can enhance the cognitive function of SAMP8 through regulating Nampt/SIRT1/FOXO3 signal pathway.关键词
通络醒脑泡腾片/SAMP8小鼠/Nampt/SIRT1/FOXO3Key words
Tongluo Xingnao Effervescent Tablets/SAMP8 mouse/Nampt/SIRT1/FOXO3分类
医药卫生引用本文复制引用
魏江平,付文君,陈欢,郑航,徐世军..通络醒脑泡腾片经Nampt/SIRT1/FOXO3途径改善SAMP8小鼠的学习记忆[J].中成药,2017,39(4):684-689,6.基金项目
“重大新药创制”科技重大专项(2013ZX09103002-008) （2013ZX09103002-008）
四川省学术和技术带头人培养资金资助项目(2015058) （2015058）
中药药理四川省青年科技创新研究团队(2014TD0007) （2014TD0007）
