中成药2017，Vol.39Issue(4)：745-750,6.DOI:10.3969/j.issn.1001-1528.2017.04.017
青钱柳质量标准的研究
Quality standard for Cyclocarya paliurus
摘要
Abstract
AIM To establish the quality standard for Cyclocarya paliurus (Batal.) ljinsk..METHODS The contents of water,ash and extract in twelve batches of samples were determined.TLC and HPLC were adopted in the qualitative identification and quantitative determination of quercetin and kaempferol,respectively,and phenol-sulfuric acid method was used for the polysaccharide content assessment.RESULTS The average contents of water,total ash,acid-insoluble ash,water-soluble extract and alcohol-soluble extract were 11.05％,5.81％,1.70％,11.25％ and 10.16％,respectively.The clear TLC spots demonstrated their strong specificity.Quercetin and kaempferol showed good linear relationships within the ranges of 0.004 953-0.022 29 mg/mL and 0.005 748-0.028 74 mg/mL (r =0.999 9),whose average recoveries were 97.1％ (RSD =2.59％) and 97.9％ (RSD =2.86％),respectively.Polysaccharide showed a good linear relationship within the range of 0.021 22-0.095 58 mg/mL,whose average recovery was 97.2％ (RSD =2.42％).The contents of three constituents in various batches of samples showed obvious differences.CONCLUSION In C.paliurus,the contents of water,total ash,acid-insoluble ash,water-soluble extract and ethanol-soluble extract should not be more than 13.0％,7.0％,2.0％,13.5％ and 12.0％,while those of quercetin,kaempferol and polysaccharide (calculated by dry product) should not be less than 0.040％,0.070％ and 0.60％,respectively.关键词
青钱柳/水分/灰分/浸出物/槲皮素/山柰酚/多糖Key words
Cyclocarya paliurus (Batal.) ljinsk./water/ash/extract/quercetin/kaempferol/polysaccharide分类
医药卫生引用本文复制引用
吴琳琳,王芳,茅向军,许乾丽..青钱柳质量标准的研究[J].中成药,2017,39(4):745-750,6.基金项目
贵州省中药材地方标准增修订项目(2015) （2015）
