中成药2017，Vol.39Issue(4)：756-760,5.DOI:10.3969/j.issn.1001-1528.2017.04.019
蜘蛛香化学成分及其神经保护活性
Chemical constituents from Valeriana jatamansi and their neuroprotective activities
摘要
Abstract
AIM To investigate the chemical constituents from Valeriana jatamansi Jones and their neuroprotective activities.METHODS The petroleum ether fraction of 75％ ethanol extract from V.jatamansi was isolated and purified by silica,Sephadex LH-20,ODS and preparative HPLC column,the structures of obtained compounds were elucidated by spectral data.Then their inhibitory effects on the apoptosis of rat pheochromocytoma (PC12) cells induced by CoCl2 were observed.RESULTS Eleven compounds were isolated and identified as bakkenolide B (1),bakkenolide D (2),jatamanvaltrate E (3),jatamanvaltrate A (4),valtrate hydrine B8 (5),valeriotetrate C (6),valtral C (7),chlorovaltrate A (8),β-sitosterol (9),β-sitosterol acetate (10),palmitic acid (11).Compounds 2,4,6 and 8 could significantly inhibit the apoptosis of PC12 cells (P ＜ 0.05).CONCLUSION Compound 2 is isolated from genus Valerianaceae for the first time,compound 10 is first obtained from this plant,and compounds 2,4,6 and 8 have neuroprotective activities.关键词
蜘蛛香/化学成分/分离鉴定/神经保护/PC12Key words
Valeriana jatamansi Jones/chemical constituents/isolation and identification/neuroprotective activities/PC12分类
医药卫生引用本文复制引用
王茹静,陈银,黄青,雍妍,张海,张仕瑾,陈雅兰,谭玉柱..蜘蛛香化学成分及其神经保护活性[J].中成药,2017,39(4):756-760,5.基金项目
国家基础科学培养基金项目(J13100340-11) （J13100340-11）
四川省教育厅重点项目(15ZA0093) （15ZA0093）
成都中医药大学科技发展基金项目(ZRQN1440) （ZRQN1440）
中药药效物质基础研究与创新中药发现四川省青年科技创新研究团队项目(2016TD0006) （2016TD0006）
四川省中医药管理局基金项目(2016Q049) （2016Q049）
