中国现代中药2016，Vol.18Issue(9)：1129-1134,6.DOI:10.13313/j.issn.1673-4890.2016.9.010
金蝉花多糖的含量组成分析及其对LPS诱导的THP-1细胞NF-κB活性的研究
Polysaccharide Content and Composition Analysis of Golden Cicada Flowers and Effect on NF-κB Activity of LPS Inducted THP-1 Cells
摘要
Abstract
Objective:To clarify golden cicada flower polysaccharide content and monosaccharide composition of polysaccharides,and study the nf-kappa B activity of THP1 cells induced by LPS.Methods:Polysaccharide from golden cicada flower was obtained by water extraction,dialysis purification,ethanol precipitation and vacuum drying,and further purified by DEAE cellulose-anion exchange column.4 kinds of purified polysaccharides were obtained.HPGPC and GC were applied to determine uronic acid content,the relative molecular mass and the monosaccharide composition.4 kinds of polysaccharides were tested for LPS induced the nf-kappa B signals of THP1 cells.Results:The neutral polysaccharide content of crude Golden cicada flower was 48.36％,the polysaccharide uronic acid content was 12.39％.The total polysaccharide content of crude Golden cicada flower was 60.75％.The relative molecular mass was 9.3 × 103 Da,molar ratio of monosaecharide composition was Xyl-Man-Gal-Glc (0.42∶0.29∶4.14∶6.38∶6.8).Polysaccharide purified by 0.2 mol.L-1 NaC1 solution elution (CSTA2) at high concentration (1000 μg·mL-1) showed the strong immune inhibitory effect,the activation rate reached only 77.2％,which was not only far lower than the positive control LPS group,and even lower than ck.High concentration (1000 μg·mL-1) and distilled water of low concentration (100 μg· mL-1)elution golden cicada flower purified polysaccharide (CSTA0) showed lower activation rate,and a good concentration dependence.Conclusion:Golden cicada polysaccharide is mainly composed of glucose (Glc),galactose (Gal),mannose (Man) and a small amount of arabinose (Ara) and xylose (Xyl).The purified polysaccharides shows THP 1 cells induced by LPS nf-kappa B inhibition.The purified polysaccharide CSTA2 and CSTA0 shows stronger immune inhibition,and deserve for further study.关键词
金蝉花/多糖/分离纯化/单糖组成/NF-κBKey words
Golden cicada flowers/polysaccharide/separation and purification/monosaccharide composition/NF-κB引用本文复制引用
杨全伟,肖柳,黄雄超,张耕,余南才,谭静玲..金蝉花多糖的含量组成分析及其对LPS诱导的THP-1细胞NF-κB活性的研究[J].中国现代中药,2016,18(9):1129-1134,6.基金项目
武汉市科技攻关项目(2013060602010263) （2013060602010263）
