中国现代中药2016，Vol.18Issue(9)：1180-1184,5.DOI:10.13313/j.issn.1673-4890.2016.9.021
ABA与CCC对南天竹离体保存的影响
Effects of ABA and CCC on Nandina domestica Thunb.in vitro Preservation
摘要
Abstract
The effects of different concentration of ABA and CCC on the growth,proliferation,survival rate and recovery growth of test-tube shoots during in vitro preservation were studied for exploring a method for in vitro preservation of Nandina domestica.The results showed that the inhibition degree of plant height by CCC treatment was deeper than ABA treatment,the inhibition effect on the proliferation was reversed,and the survival rate(83.33％)by CCC treatment was obviously higher than ABA treatment (56.67％).So the suitable medium for N.domestica in vitro preservation was MS + 30 g·L-1 sucrose + 0.50 mg·L-1 6-BA+0.05 mg·L-1NAA + 5 mg·L-1 CCC,on which in vitro preservation for 120 d,the survival rate,proliferation coefficient and plant height was 83.33％,2.33 and 1.59 cm,respectively.After in vitro preservation for 120 d,the survival rate on recovery culture reached 96.67％,and their morphology was normal.By adding CCC,the growth of test-tube shoots was alleviated which could applicate for N.domestica germplasm in vitro preservation.关键词
南天竹/离体保存/CCC/ABAKey words
Nandina domestica/in vitro preservation/CCC/ABA引用本文复制引用
蔡鹏,吴婵榕,刘华英,唐娅娜..ABA与CCC对南天竹离体保存的影响[J].中国现代中药,2016,18(9):1180-1184,5.基金项目
国家自然科学基金(31160071) （31160071）
