中国现代中药2016，Vol.18Issue(9)：1196-1200,5.DOI:10.13313/j.issn.1673-4890.2016.9.025
大孔树脂分离纯化西洋参叶总皂苷的工艺研究
Purification of Total Saponins of Leaves of Panax quinquefolius by Macroreticular Resin
摘要
Abstract
Objective:The study aims at purification of total saponins of leaves of Panax quinqtuefloius by D-101 macroreticular resin.Methods:Using the adsorption and desorption rates of total saponins and ginsenside Rb3 as parameters,the purification technological conditions of total saponins of the leaves of P.quinquefloius by macroreticular resin were experimentally investigated.The curves of adsorption kinetics and dynamic adsorption kinetics of macroreticular resin were exploited.Results:The rate of medicinal materials and the wet resin content was 3:10.The flow rate of the macro-reticular resin column was 1.5 mL· min-1.The experiments were repeated twice.The column was eluted by water of three times volume and ethanol of three times volume.After then ethanol elution was collected.The ethanol elution was concentrated,dried,and then the total saponins extract of the leaves of P.quinquefloius was obtained.The contents of total saponins in extract was more than 75％.The contents of ginsenside Rb3 was more than 10％.The yield of total saponins extract was 14％.Conclusion:This method is simple,repeatable,available for extract and purification of total saponins of the leaves of P.quinquefloius.关键词
西洋参叶/大孔吸附树脂/总皂苷/人参皂苷Rb3/工艺参数Key words
Leaves of Panax quinquefloius/macroreticular resin/total saponins/ginsenside Rb3/technological conditions and parameters引用本文复制引用
郭婷婷,王兆华,张大军..大孔树脂分离纯化西洋参叶总皂苷的工艺研究[J].中国现代中药,2016,18(9):1196-1200,5.基金项目
国家科技重大专项(2013ZX09103002-022) （2013ZX09103002-022）
吉林省科技发展计划专项(20140311014YY) （20140311014YY）
