中国现代中药2016，Vol.18Issue(9)：1204-1208,5.DOI:10.13313/j.issn.1673-4890.2016.9.027
HPLC法测定市售天麻饮片中6种活性成分的含量
Determination of 6 Active Components in Commercially Available Gastrodiae Rhizoma by HPLC
摘要
Abstract
Objective:To determine 6 active components (gastrodin,4-hydroxybenzyl alcohol,vanillyl alcohol,parishin A,parishin B,parishin C) of Gastrodiae Rhizomaby HPLC.Methods:The separation was carried out by an Hypersil Cl8 column (250 mm × 4.6,5 μm) in a mobile phase consisting of 0.5％ CH3COOH-H2O(A) and 0.5％ CH3COOH-CH3 OH (B) with a gradient elution system (0→10 min,98 ％ A;10→60 min,98％ →60％ A;and 60→75 min,60％ A).Detection wavelength was set at 270 nm and column temperature was 30 ℃.Injection volume was 20 μL.Results:The six components had good linearity (r ≥ 0.999 3).The average recovery rates were 96.99％ ～ 101.73％ with RSD ＜ 2.5％.Gastrodiae Rhizoma of 29 different batches was determined,in which Gastrodiae Rhizoma from Yunnan and Sichuan areas had the highest content of gastrodin.The contents of 6 components in Gastrodiae Rhizoma varied in different areas.The contents of parishin A was the highest while the contents of 4-hydroxybenzyl alcohol and vanillyl alcohol were lower.Conclusion:The method is rapid,simple,accurate and can be used to control quality of Gastrodiae Rhizoma in a more comprehensive and effective way.关键词
天麻/高效液相色谱/含量测定/质量控制Key words
Gastrodiae Rhizoma/HPLC/determination/quality control引用本文复制引用
刘思婷,单鸣秋,郭舒臣,钱岩,张丽..HPLC法测定市售天麻饮片中6种活性成分的含量[J].中国现代中药,2016,18(9):1204-1208,5.基金项目
江苏省中药资源产业化过程协同创新中心项目(ZDXM-1-3) （ZDXM-1-3）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
