安徽农业科学2017，Vol.45Issue(10)：1-4,47,5.
响应面法优化单酶复配酶解辐照玉米秸秆的研究
Optimization of Enzyme Complex Formulation of Irradiation Corn Straw by Response Surface Method
摘要
Abstract
[Objective] To study the optimal ratio of enzyme complex formulation.[Method] 1 200 kGy 60Co γ-ray irradiation corn straw as raw material,the response surface methodology was applied for analyzing the cellobiohydrolase,the endoglucanase,the β-glucanase and the xylanase.[Result] The reducing sugar content was extremely significantly by xylanase dosage but not significantly impacted by cellobiohydrolase,β-glucanase and endoglucanase.The optimum conditions for supplemental levels were found to be hydrolysis at cellobiohydrolase ratio of 1.07 U/g,endoglucanase ratio of 31.53 U/g,β-glucanase ratio of 20.81 U/g and xylanase ratio of 81.96 U/g.The reducing sugar content under these conditions was 372.624 mg/g than before optimization and basically according with the model prediction value.[Conclusion] The optimization of enzyme complex formulation of irradiation corn straw by central composite design and response surface method is reliable,and it can provide reference for the complex of single enzyme.关键词
γ-射线辐照/响应面法/酶解/葡萄糖Key words
γ-ray irradiation/Response surface method/Hydrolysis/Glucose分类
农业科技引用本文复制引用
滕彦淇,胡秋龙,苏小军,李会东,金元昌..响应面法优化单酶复配酶解辐照玉米秸秆的研究[J].安徽农业科学,2017,45(10):1-4,47,5.基金项目
湖南省科技重大专项(2015NK1001-4) （2015NK1001-4）
湖南省教育厅重点项目(15A067) （15A067）
生物工程专业省级校企合作人才培养示范基地项目(G145813). （G145813）
