安徽农业科学2017，Vol.45Issue(10)：126-128,183,4.
水稻RSSG58的进化分析及其拟南芥同源基因ATG58的组织表达模式
Evolutionary Analysis of Rice RSSG58 and Tissue Expression Pattern Analysis of Its Arabidopsis Homologous Gene ATG58
摘要
Abstract
[Objective] To find the homologous gene of rice RSSG58 in Arabidopsis thaliana and observe its tissue expression pattern.[Method] The phylogenetic tree of rice RSSG58 was constructed by using the related gene informatics website.The homologous gene ATG58::GUS locus was constructed into A.thaliana to observe and analyze the tissue expression pattern of ATG58.[Result] The phylogenetic tree analysis and sequence comparison showed that ATG58 had a highest sequence similarity with RSSG58.GUS locating genetic material showed that ATG58 was expressed in various tissues.The expression of ATG58 was relatively higher in root,cotyledon,hypocotyl and embryo of seedling stage,and it was superior to the buds and anthers in the reproductive development stage.ATG58 had similar GUS localization expression pattern with RSSG58.[Conclusion] ATG58 is the homologous gene of rice RSSG58,which is consistent with RSSG58 in the tissue expression pattern.These results provide a basis for further study of the role and mechanism of A.thaliana ATG58 and rice RSSG58 in the development of male gametes.关键词
水稻/RSSG58/ATG58/β-葡萄糖苷酸酶(GUS)/组织表达/进化树分析Key words
Oryza saliva L/RSSG58/ATG58/Beta-glucuronidase(GUS)/Tissues expression/Phylogenetic tree analysis分类
农业科技引用本文复制引用
于东明,李筱雨,孙申领,孙炎锋,王伟,吕东,苗琛..水稻RSSG58的进化分析及其拟南芥同源基因ATG58的组织表达模式[J].安徽农业科学,2017,45(10):126-128,183,4.基金项目
国家自然科学基金项目(30771315). （30771315）
