草业学报2017，Vol.26Issue(2)：69-77,9.DOI:10.11686/cyxb2016133
镉污染和接种丛枝菌根真菌对紫花苜蓿生长和氮吸收的影响
Effects of cadmium and arbuscular mycorrhizal fungi inoculation on the growth and nitrogen uptake of alfalfa (Medicago sativa)
摘要
Abstract
A pot experiment in a greenhouse was conducted to investigate the growth and nitrogen (N) uptake of Medicago sativa inoculated with different arbuscular mycorrhizal fungi [Glomus aggregatum (Ga),Glomus etunicatum (Ge),Glomus intraradices (Gi),Glomus tortuosum (Gt),Glomus versiforme (Gv)] or un-inoculated in soil without or with cadmium at two concentrations (6 and 12 mg Cd/kg).Mycorrhizal colonization of alfalfa inoculated with Ga,Gi,and Gt was 33.90％,19.17％,and 31.95％ lower,respectively,in 12 mg Cd/kg soil than in 0 mg Cd/kg soil.In soil without Cd,the biomass of the Gt-inoculated plants was 33.19 ％,64.74％,57.29％,and 34.91％ higher than that in the Ga,Ge,Gi,and Gv treatments,respectively.In 12 mg Cd/kg soil,the biomass of the Gt-inoculated plants was 16.67％,34.07％,32.96％,and 52.76％ lower than that of those inocluated with Ga,Ge,Gi,and Gv,respectively.Compared with uninoculated plants,those inoculated with Gv showed significantly increased shoot height,number of nodules,shoot biomass,total biomass,shoot N content,and total N content (by 65.41％,95.24％,61.87％,50.30％,5.83％,and 71.55％,respectively) in soil containing 12 mg Cd/kg.The NH4+-N and NO3--N concentrations in soil significantly decreased with increasing Cd levels in the Gv-inoculation treatments.These results showed that Gv inoculation promoted the growth and N uptake of alfalfa in Cd-contaminated soils,whereas Gt inoculation could not benefit the growth of alfalfa grown in soils containing more than 6 mg Cd/kg.关键词
紫花苜蓿/丛菌根真菌/镉/氮吸收Key words
alfalfa/arbuscular mycorrhizal fungi/cadmium/nitrogen uptake引用本文复制引用
刘芳,景戍旋,胡健,肖燕,张英俊..镉污染和接种丛枝菌根真菌对紫花苜蓿生长和氮吸收的影响[J].草业学报,2017,26(2):69-77,9.基金项目
中央高校基本科研业务专项资金(KYZ201554),国家自然科学基金(31501996)和江苏省自然科学基金(BK20150665)资助. （KYZ201554）
