草业学报2017，Vol.26Issue(2)：78-87,10.DOI:10.11686/cyxb2016111
紫花苜蓿根腐病原——厚垣镰刀菌的鉴定及其拮抗菌的筛选
Identification of alfalfa root rot caused by Fusarium chlamydosporum and screening of antagonistic bacterial strains
摘要
Abstract
To identify the causal pathogens of alfalfa root rot with strong pathogenicity,pathogens were isolated from alfalfa (Medicago sativa) plants with alfalfa root rot growing in Wuwei City,Gansu Province.The pathogens were identified based on their morphology and by molecular methods.The results showed that the strain MXGF7 caused acute root rot of alfalfa.Most of its macroconidia were fusiform or sickle-shaped (3.3-4.7 μm × 14.8-32.0 μm),and its microconidia were fusiform or dumbbell-shaped (2.9-4.2 μm × 8.4-18.2um).The ITS sequence and the Fu3/Fu4 specificity gene sequence of the pathogenic fungus shared 99 ％ similarity with those of Fusarium chlamydosporum,and so the pathogenic fungus was identified as F.chlamydosporum.We isolated 123 endophytic bacterial strains from alpine grassland in the Eastern Qilian Mountains,and evaluated their ability to antagonize the pathogenic strain of F.chlamydosporum.Sixty-one of the bacterial strains showed an antifungal ratio greater than 55％.The most antagonistic bacterium,strain 265ZY3,was identified as Bacillus amyloliquefaciens based in its morphology and 16S rDNA gene sequence.关键词
苜蓿根腐病/厚垣镰孢菌/内生细菌/鉴定Key words
alfalfa root rot/Fusarium chlamydosporum/endophytic bacteria/identification引用本文复制引用
柏玉晶,姚玉玲,张振粉,杨成德,薛莉..紫花苜蓿根腐病原——厚垣镰刀菌的鉴定及其拮抗菌的筛选[J].草业学报,2017,26(2):78-87,10.基金项目
国家自然科学基金(No.31460639),草业生态系统教育部重点实验室(甘肃农业大学)开放课题项目(CYzs-2011011),甘肃省农牧厅科技支撑项目和甘肃省自然科学基金(145RJZA130)资助. （No.31460639）
