森林与环境学报2017，Vol.37Issue(2)：129-135,7.DOI:10.13324/j.cnki.jfcf.2017.02.001
杉木不同器官愈伤组织和再生芽的诱导效率
Efficiency of callus and regeneration bud induction from different explants of Cunninghamia lanceolata
摘要
Abstract
For the purpose of establishing callus induction and bud regenerate system for Cunninghamia lanceolata (Lamb.) Hook.,and proving an effective receptor procedure for researching into the genetic engineering of somatic hybridization and genetic transformation.Using immature embryo,hypocotyls and cotyledons of seedlings and stems of sterile seedlings as explants,the effect of the following factors including basal medium,different types of hormone concentrations and combinations on callus induction and regeneration buds differentiation were investigated respectively.The results indicated that different hormone combinations on callus induction of different explants have a significant influence.The optimal medium for immature embryo induction callus was MS+2,4-D 2 mg·L-1 +6-BA 1 mg·L-1 +KT 1 mg·L-1,the induction rate was 92.7％;the optimal medium for cotyledons and hypocotyls induction callus was MS+6-BA 2 mg·L-1+NAA 2 mg·L-1+TDZ 0.01 mg·L-1,the induction rates were 97.4％ and 95.2％ respectively;the optimal medium for stems from sterile seedlings induction callus was MS+2,4-D 1 mg·L-1+NAA 2 mg·L-1 +TDZ 0.01 mg·L-1,the induction rate was 86.7％;the optimal medium for callus induction regeneration bud was MS+6-BA 0.5 mg· L-1 +KT 1.5 mg· L-1,the induction rate was 80％.The callus with loose texture has the highest regeneration rate (65％),but for a single callus with homogeneous texture,its maximum number of regenerated buds was 6.The basic medium has great influence on the texture of callus that the callus induced by 1/2MS basal medium showed loose texture,homogeneous surface with visible granular cell for MS basal medium,and dense callus for DCR basal medium.关键词
杉木/愈伤组织/未成熟胚/子叶/下胚轴/组培苗茎段Key words
Cunninghamia lanceolata (Lamb.) Hook./callus/immature zygotic embryo/cotyledons/hypocotyls/sterile seedings stems分类
农业科技引用本文复制引用
黄娟,吴鹏飞,李惠通,马祥庆..杉木不同器官愈伤组织和再生芽的诱导效率[J].森林与环境学报,2017,37(2):129-135,7.基金项目
国家自然科学基金项目(U1405211) （U1405211）
国家科技支撑计划项目(2015BAD09B010102). （2015BAD09B010102）
