森林与环境学报2017，Vol.37Issue(2)：207-211,5.DOI:10.13324/j.cnki.jfcf.2017.02.013
高产纤维素酶菌株的鉴定及其活性特征
Identification of strains producing cellulase and its enzyme activity determination
摘要
Abstract
Based on cumulation cultivation,filter paper disintegrating,carboxymethyl cellulose (CMC) enzyme activity determination,strains with great capacity in cellulose decomposition were isolated to analyze the CMC enzyme activities of fallen logs in the Tsuga longibracteata W.C.Cheng forest of Tianbaoyan National Nature Reserve.18S rRNA sequencing analysis of the selected stains was carried out and identified as Penicillium spinulosum which the CMC enzyme activity was 243.73 U·mL-1.The P.spinulosum strain had the ability in cellulose degradation in the T.longibracteata forest of Tianbaoyan National Nature Reserve.关键词
纤维素降解菌/酶活性/倒木/长苞铁杉Key words
cellulose-decomposing microorganisms/enzyme activity/fallen log/Tsuga longibracteata W.C.Cheng分类
农业科技引用本文复制引用
简立燕,何东进,刘进山,洪伟,游巍斌,肖石红..高产纤维素酶菌株的鉴定及其活性特征[J].森林与环境学报,2017,37(2):207-211,5.基金项目
国家自然科学基金项目(31370624) （31370624）
教育部博士科学点基金项目(20103515110005) （20103515110005）
福建省自然科学基金项目(08J0116,2011J01071). （08J0116,2011J01071）
