森林与环境学报2017，Vol.37Issue(2)：212-217,6.DOI:10.13324/j.cnki.jfcf.2017.02.014
金龟子绿僵菌对椰心叶甲的致病力
Pathogenicity of Metarhizium anisopliae against Brontispa longissima
摘要
Abstract
The virulence of 5 Metarhizium anisopliae strains against Brontispa longissima in different development stages were determined by spraying.The results showed that various strains of M.anisopliae had certain virulence against B.longissima after inoculation with (1±0.5)x107 spore·mL-1 for 10 d.Compared various strains,Ma1775 strain exhibited the most effective virulence against B.longissima.Mter treatment,the Ma1775 strain caused a cumulative corrected morality of (67.96±3.66)％,the cadaver rate of (60.59±1.31)％ and the median lethal time (LT50) of (7.11±0.52) d in adults.For 5 instar larvae,the corrected mortality and cadaver rate were (78.53±2.73)％ and (73.55±3.22)％,with LT50(5.77±0.38) d.The corrected mortality and cadaver rate were (82.59±2.48)％ and (77.86±3.44)％,and LT50 was (5.80±0.14) d for 4 instar larvae.The corrected mortality and cadaver rate were (73.46±4.60)％ and (69.50±3.87)％ for 2～3 instar larvae,with LT50(6.49±0.12) d.The median lethal concentration (LC5o) of Ma1775 strain at each development stage in 6 d were 3.52×10s,7.13×107,2.48× 107,7.69×107 spore· mL-1,respectively.By employing a time-dose-mortality model to estimate parameters of time and dose,the Ma1775 strain had a high lethal effect on B.longissima during 3～7 d after treatment.Thus,the Ma1775 strain has been proved to be a great potential application in biological control to B.longissima.关键词
金龟子绿僵菌/椰心叶甲/致病力/菌株筛选Key words
Metarhizium anisopliae (Metschnikoff)/Brontispa longissima (Gestro)/pathogenicity/strain screening分类
农业科技引用本文复制引用
徐超,童应华..金龟子绿僵菌对椰心叶甲的致病力[J].森林与环境学报,2017,37(2):212-217,6.基金项目
厦门市科技计划项目(2014S0172) （2014S0172）
福建省自然科学基金面上项目(2012D083) （2012D083）
福建省林业厅林业科研专项计划项目[闽林科(2010)4号]. （2010）
