分析化学2017，Vol.45Issue(5)：633-640,8.DOI:10.11895/j.issn.0253-3820.170020
利用代谢组学研究大气细颗粒物的生殖毒性效应
Study on Reproductive Toxicity of Fine Particulate Matter by Metabolomics
摘要
Abstract
Air born fine particulate matter (PM2.5) pollution is a serious environmental problem, and it is very important to study the toxicity effect and mechanism of PM2.5.In this study, a liquid chromatography-mass spectrometric (LC-MS) method on the basis of metabolomics technique was developed to investigate the metabolic disruption effect and reproductive toxicity mechanism of PM2.5 on rat testis.The profile of aqueous and organic testis metabolic extracts were acquired, and then analyzed by partial least square-discriminant analysis and nonparametric test.The results showed that control group and treatment group were clearly discriminated in the scoring plot of PLS-DA models, indicating PM2.5 treatment induced significant difference in testis metabolome.A total of 56 differential metabolites were identified, and further pathway analysis suggested that PM2.5 exposure induced amino acid and nucleotide metabolism disorder, steroid hormone metabolism imbalance and abnormal lipid metabolism.These important pathways may be the key molecular events in the PM2.5 reproductive toxicity.关键词
代谢组学/大气细颗粒物/睾丸/生殖毒性/液相色谱-质谱Key words
Metabolomics/Fine particulate matter/Testis/Reproductive toxicity/Liquid chromatography-mass spectrometry引用本文复制引用
王晓飞,蒋守芳,张维冰,张凌怡,刘颖,杜晓妍,张洁,申河清..利用代谢组学研究大气细颗粒物的生殖毒性效应[J].分析化学,2017,45(5):633-640,8.基金项目
本文系国家自然科学基金(Nos.21177123, 21311130119, 21407143, 21677141)、宁波市科技计划项目(No.2014A610284)、中国科学院城市环境研究所青年人才领域前沿项目(No.IUEZD201401)和中国科学院青年促进会基金(No.CAS2015246) 资助 This work was supported by the National Natural Science Foundation of China (Nos.21407143, 21677141) （Nos.21177123, 21311130119, 21407143, 21677141）
