分析化学2017，Vol.45Issue(5)：641-647,7.DOI:10.11895/j.issn.0253-3820.160748
基于脂质代谢组学研究褐藻糖胶对黄颡鱼幼鱼的影响
Effects of Fucoidan on Lipidomics Profiling of Juvenile Yellow Catfish (Pelteobagrus fulvidraco) during Feeding Time
摘要
Abstract
The effects of fucoidan on the lipidomics profiling of juvenile yellow catfish (pelteobagrus fulvidraco) during different feeding time (week 1, week 2, week 3 and week 8) were investigated by ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) combined with multivariate data analysis, e.g.principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA).Based on VIP and p values, 11 lipid biomarkers were screened including lysophosphatidylcholine (Lyso-PC) 16∶0, phosphatidylcholine (PC) 22∶6/16∶0, phosphatidylethanolamine (PE) 22∶6/16∶0, phosphatidylinositol (PI) 18∶0/22∶6, diacylglycerol (DAG) 16∶0/16∶0,DAG 16∶0/18∶1, DAG 18∶1/18∶1, triglycerides (TAG) 20∶5/16∶0/18∶3, TAG 20∶4/16∶1/18∶2, TAG 16∶0/18∶2/20∶5 and TAG 18∶2/14∶0/18∶1.It was found that Lyso-PC, PC, PE and PI had the largest levels at the eighth week;the levels of DAG were decreased at the second and fourth weeks, and increased at the eighth week.While TAG was different: the content of TAG 18∶2/14∶0/18∶1, TAG 20∶5/16∶0/18∶3 and TAG 20∶4/16∶1/18∶2 increased in response to fucoidan, but TAG 16∶0/18∶2/20∶5 decreased little.Therefore, for juvenile yellow catfish, fucoidan can affect its lipid metabolism, which provides a theoretical basis for investigation of the influence of fucoidan on the response mechanism of lipid metabolism of juvenile yellow catfish.关键词
黄颡鱼/褐藻糖胶/超高效液相色谱-四极杆-飞行时间质谱/脂质组学Key words
Pelteoobagrus fulvidraco/Fucoidan/Ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry/Lipidomic引用本文复制引用
徐盼盼,宋悦,陈娟娟,杨锐,严小军,钟琪..基于脂质代谢组学研究褐藻糖胶对黄颡鱼幼鱼的影响[J].分析化学,2017,45(5):641-647,7.基金项目
本文系浙江省自然科学基金(No.LY17D060002) （No.LY17D060002）
浙江省科技厅公益项目(分析测试)(No.2016C37090),宁波大学李达三叶耀珍伉俪李本俊海洋生物医药发展基金， 国家111引智基地和宁波大学王宽诚幸福基金资助 This work was supported by the Natural Science Foundation of Zhejiang Province, China (No.LY17D060002), the Science Technology Department of Zhejiang Province (No.2016C37090), Li Dak Sum Yip Yio Chin Kenneth LiMarine Biopharmaceutical Development Fund, National 111 Project of China and K.C.Wong Magna Fund in Ningbo University. （分析测试）
