广东医学2017，Vol.38Issue(5)：692-695,4.
多探针荧光原位杂交技术联合G显带核型分析诊断维吾尔族成人急性髓系白血病的效果
Diagnostic utility of Multiprobe Fluorescence in situ Hybridization combined with G-banding chromosome analysis in Uyghur Adult Patients with newly diagnosed Acute Myeloid Leukemia in Kashgar region
摘要
Abstract
Objective To explore the clinical application of multiprobe fluorescence in situ hybridization (FISH) combined with conventional cytogenetic G-banding(CCG) analysis in the diagnosis of acute myeloid leukemia (AML) in Uyghur adult patients in Kashgar region.Methods The multiprobe AML panel comprising 8 different FISH probes for PML/RAR transfusion gene,AML1/ETO transfusion gene,CBF/MYH11 transfusion gene,MLL gene,Del(20q),-7/ Del(7q),Del(5q),and P53 deletion was performed in 30 Uyghur adult patients newly diagnosed with AML in Kashgar region.The multiprobe FISH results were compared with those of CCG.Results With the multiprobe FISH panel,cytogcnetic aberrations were detected in 22 cases (22/30,66.7％),which have involved PML/RAR transfusion gene,AML1/ETO transfusion gene,CBF/MYH11 transfusion gene,MLL gene,trisomy 8,-7/Del (7q),Del (5q),P53 deletion.CCG was only idcntificd in 9 cases with the corresponding cytogenetic abnormalities and 2 cases with other cytogenetic abnormalities.The positive rate of multiprobe FISH was significantly higher than the CCG (P ＜ 0.05),and the detection rate by the combination of FISH and CCG increased to 73.3％.Conclusion Comparing with CCG,multiprobe FISH panel is more accurate,effective and rapid in the diagnosis of AML with cytogenetic aberrations.Multiprobe FISH combined with CCG can improve the detection rate of cytogenetic aberrations in AML and can provide more objective and reliable evidences for the diagnosis of Uyghur newly diagnosed adult AML in Kashgar region.关键词
多探针荧光原位杂交/G显带核型分析/细胞遗传学异常/急性髓系白血病/维吾尔族Key words
multiprobe fluorescence in situ hybridization/cytogentic G-banding/cytogenetic aberration/acute myeloid leukemia/Ugyhur引用本文复制引用
黄竞,周长华,阿依姆妮萨·阿卜杜热合曼,高腾腾,海热萨·阿不力米提,刘志,穆尼热·艾尼,徐丽,艾克拜尔·阿布都热衣木..多探针荧光原位杂交技术联合G显带核型分析诊断维吾尔族成人急性髓系白血病的效果[J].广东医学,2017,38(5):692-695,4.基金项目
广东省科技计划项目(编号:2015A020210068),广东省第二人民医院青年基金项目(编号:YQ2015-012,YQ2015-005) （编号:2015A020210068）
