临床与病理杂志2017，Vol.37Issue(2)：257-263,7.DOI:10.3978/j.issn.2095-6959.2017.02.007
红景天苷对C6胶质瘤细胞系迁移及生长的影响
Effects of salidroside on migration and growth of C6 glioma cell line
摘要
Abstract
Objective:To investigate the effects of Salidroside on migration and growth of C6 glioma cell line.Methods:CCK-8 assay and LDH assay were applied to determine the optimal effect concentration of salidroside on C6 glioma cells.Cell growth curve,Cell morphology and Wound scratch assay were carried out to illustrate the influence of salidroside on migration and growth of C6 glioma cells.The expression levels of MMP-9 and Cyclin A in C6 glioma cells were tested by Western blot and qPCR.Results:CCK-8 assay and LDH assay demonstrated that within the scope of 12.5,25,50,100 and 200 tmol/L,100 tmol/L is the optimal effect concentration of salidroside on C6 glioma cells.Cell growth curve and Cell morphology showed that the growth of C6 glioma cells was suppressed obviously after treated with salidroside.Wound scratch assay revealed that the invasiveness of C6 glioma cells had been attenuated in the SLDS group,and Western blot and qPCR demonstrated that the expression levels of MMP-9 and Cyclin A in the SLDS group were evidently lower than the control group.Conclusion:Salidroside inhibits the growth and migration of C6 glioma cell line.关键词
红景天苷/胶质瘤/基质金属蛋白酶9/周期蛋白AKey words
salidroside/glioma/matrix metalloproteinase 9/Cyclin A引用本文复制引用
刘宽,赵富花,张国庆,曾现伟..红景天苷对C6胶质瘤细胞系迁移及生长的影响[J].临床与病理杂志,2017,37(2):257-263,7.基金项目
山东省自然科学基金(ZR2013HM039).This work was supported by Natural Science Foundation of Shandong Province (ZR2013HM039),P.R.China. （ZR2013HM039）
