临床与病理杂志2017，Vol.37Issue(2)：331-338,8.DOI:10.3978/j.issn.2095-6959.2017.02.019
姜黄素治疗聚乙烯颗粒诱导骨溶解的体内实验
In vivo experiment of curcumin treating polyethylene particle induced osteolysis
摘要
Abstract
Objective:To investigate the therapeutic effect and mechanism of curcumin on polyethylene particle induced osteolysis,and provide new insights into the prevention and treatment of prosthetic aseptic loosening.Methods:The mouse models of bone graft air pouch were established and randomly divided into the following four groups:blank group,polyethylene (PE) group,solvent group,curcumin group (curcumin + solvent).After the intervention,all the mice were harvested and the air pouchs and skulls were taken out.Tartrate-resistant acid phosphatase (TRAP) staining was used to detect the amount of osteoclast in skull.Q-PCR was performed to measure the mRNA level of c-Fos,NFATc 1;Westen blot was performed to test the protein level of IκBα and p-IκBα in air pouch tissue.Results:The TRAP staining result showed that dyed osteoclast in polyethylene and solvent group were more than that in the other two groups;however,blank and curcumin group showed little or no osteoclast dyeing.QPCR:the mRNA level of c-Fos,polyethylene group ＞ solvent group ＞ blank group ＞ curcumin group,compared the former two groups with later two,the difference was statistically significant (P＜0.01);the mRNA level of NFATcl,polyethylene group ＞ solvent group ＞ blank group ＞ curcumin group,compared the former two groups with later two,the difference was statistically significant (P＜0.01).Western blot:the IκBa protein grey value,curcumin group ＞ blank group ＞ solvent group ＞ polyethylene group,compared the former two groups with later two,the difference was statistically significant (P＜0.01);p-IκBα protein grey value,polyethylene group ＞ solvent group ＞ blank group ＞ curcumin group,compared the former two groups with later two,the difference was statistically significant (P＜0.01).Conclusion:In air pouch bone graft model of mice,curcumin could reduce ultra-high molecular weight polyethylene induced bone dissolution reaction by inhibiting NF-κB activation and c-FOS,NFATc1 expression.关键词
骨溶解/无菌性松动/姜黄素/小鼠气囊植骨模型/NF-1κB/c-Fos/NFATc1/NF-1cB抑制蛋白/磷酸化NF-κB抑制蛋白Key words
osteolysis/aseptic loosening/curcumin/air pouch model/NF-κB/c-Fos/NFATc1/IκBα/p-IκBα引用本文复制引用
韩斐,胡懿邻,安森博,雷鹏飞,汪龙..姜黄素治疗聚乙烯颗粒诱导骨溶解的体内实验[J].临床与病理杂志,2017,37(2):331-338,8.基金项目
国家自然科学基金(81601883) （81601883）
湖南省卫生计生委科研基金(B2015-005). This work was supported by the National Nature Science Foundation (81601883),Scientific Research Foundation of Health and Family Planning Commission of Hunan Province (B2015-005),P.R.China. （B2015-005）
