临床与病理杂志2017，Vol.37Issue(2)：392-397,6.DOI:10.3978/j.issn.2095-6959.2017.02.028
miR-124对C6胶质瘤细胞增殖和迁移能力的影响
Effect of miR-I24 on proliferation and migration of C6 glioma cells
摘要
Abstract
Objective:To investigate the effect of miR-124 on the proliferation and migration of glioma C6 cells in vitro.Methods:The glioma C6 cells cultured in vitro were divided into three groups as following an miR-124 mimic group,a negative control group and a blank control group.The expression level of miR-124 was detected by RTPCR.Growth curves were drawn.MTT assay was used to determine the proliferation ability of C6 cells.The inhibition rate was calculated.Scratch migration assay was used to determine the migration ability of C6 cells.The mobility was calculated.Results:According to the growth curves,the number of glioma C6 cells in miR124 mimic group was significantly less than those in the negative control group on the third day after transfection (5.410±0.463 vs.6.917±0.385;P＜0.01).Compared to the negative control group and the blank control group,the expression ofmiR-124 was higher in the miR-124 mimic group (5.92±0.56 vs.0.93±0.13 and 1.00±0.12;P＜0.01).According to MTT assay,miR-124 significantly inhibited the proliferation ability of C6 glioma cells,the inhibition rate in the miR-124 mimic group was significantly higher than that in the negative control group (42.90±5.169 vs.10.24±3.351;P＜0.01).According to Scratch migration assay,miR-124 inhibited the migration ability of C6 glioma cells,the migration rate in the negative control group was significantly higher than that in the miR-124 mimic group (98.79±1.210 vs.81.72±5.972;P＜0.05).Conclusion:MiR-124 can significantly inhibit the proliferation and migration of C6 glioma cells.关键词
miR-124/C6胶质瘤细胞/增殖/迁移Key words
miR-124/C6 glioma cells/proliferation/migration引用本文复制引用
王磊,张国庆,赵富花,左苗苗,曾现伟..miR-124对C6胶质瘤细胞增殖和迁移能力的影响[J].临床与病理杂志,2017,37(2):392-397,6.基金项目
国家自然科学基金(8167021239) （8167021239）
国家科技部科技支撑计划(2015BAI06B05) （2015BAI06B05）
山东省科技发展计划项目(2015GGB14296). This work was supported by the National Natural Science Foundation (8167021239),National Science and Technology Support Program (2015BAI06B05),and Science and Technology Development Project of Shandong Province (2015GGB14296),P.R.China. （2015GGB14296）
