甘蔗糖业Issue(2)：1-8,8.
EMS诱变甘蔗愈伤组织的初步研究
Mutagenesis Application to Sugarcane (Saccharum Spp.) Embryogenic Callus with EMS
摘要
Abstract
In order to find out the optimum dose and time period of ethyl methyl sulphonate (EMS), with which the chemical mutagenesis was applied in sugarcane by treating the embryogenic callus, two sugarcane cultivars (ROC22 and YT93-159) were induced throughin vitro mutagenesis with varying doses (8, 24, 40 μmol/L) and time periods (exposed for 2, 4, 6 h) of EMS. And the proper medium was also found out by the embryo germination experiments. The results showed that for both the cultivars, embryo germination was well on the medium containing Murashige and Skoog medium (MS) basal salts and vitamins, 2.0 mg/L KT, 2.0 mg/L NAA, 3.0 percentage (w/v) sucrose, and 0.8 percentage (w/v) agar. Based on some factors such as embryogenic callus proliferation and embryo germination, 24 μmol/L for 2 h treatment or 8 μmol/L for 6 h treatment was optimum.关键词
甘蔗/化学诱变/甲基磺酸乙酯/愈伤组织Key words
Saccharumspp./Chemical mutagenesis/Ethyl methyl sulphonate (EMS)/Embryogenic callus分类
农业科技引用本文复制引用
何慧怡,樊丽娜,齐永文,劳方业,陈勇生,黄忠兴,胡斐..EMS诱变甘蔗愈伤组织的初步研究[J].甘蔗糖业,2017,(2):1-8,8.基金项目
国家甘蔗产业技术体系岗位科学家(CARS-20-1-4) （CARS-20-1-4）
广东省科技计划(2014B070705002、2014B090907006、2015A020209026、2016A030313415、2016A030303015) （2014B070705002、2014B090907006、2015A020209026、2016A030313415、2016A030303015）
