华北农学报2017，Vol.32Issue(1)：36-40,5.DOI:10.7668/hbnxb.2017.01.006
大白菜干烧心病抗性QTL定位研究
Study on Tipburn Resistance QTL Mapping in Chinese Cabbage
摘要
Abstract
Different tipburn resistance materials of Chinese cabbage were used as materials to study the inheritance of resistance gene to tipburn and to conduct QTL mapping so as to provide a theoretical basis for the molecular assistant selection (MAS) breeding and resistance mechanism.This research adopted significantly different resistance tipburn line high inbred Qingmaye type Black 227 (resistant lines) and high inbred Baotou type B120 (susceptible lines)as materials to obtain DH group by microspore culture F1 generation.We classified degrees of the disease and combined the molecular genetic map of Chinese cabbage.According to disease severity in the field of DH group,QTL analysis on tipnurn resistance genes were conducted by MapQTL 5.0 software.Results showed that the inheritance of the resistance gene to Chinese cabbage tipburn in tested materials fit to the inheritance law of quantitative trait.This study detected two InDel markers BrID10343 and BrID10349 which link closelyed with tipburn resistance gene of Chinese cabbage.Two markers were on the Chr.7,in which the genetic distance of 1.031 cM and genetic contribution rates were above 40％.InDel markers BrID10343 and BrID10349 linked closelyed with tipburn resistance gene of Chinese cabbage.The results would be of great benefit to fine mapping for the major QTL of tipburn resistance gene,also the results will lay a good foundation for Chinese cabbage MAS resistance breeding.关键词
大白菜/干烧心病/QTL定位Key words
Chinese cabbage/Tipburn/QTL mapping分类
农业科技引用本文复制引用
金秀卿,张斌,李梅,闻凤英,刘晓晖,王超楠,黄志银..大白菜干烧心病抗性QTL定位研究[J].华北农学报,2017,32(1):36-40,5.基金项目
国家重点研发计划项目(2016YFD0101701) （2016YFD0101701）
天津市自然科学基金项目(16JCYBJC29300) （16JCYBJC29300）
国家大宗蔬菜产业技术体系天津综合试验站(CARS-25-G-02) （CARS-25-G-02）
“农业科研杰出人才培养计划”项目 （）
