华东理工大学学报（自然科学版）2017，Vol.43Issue(2)：184-192,9.DOI:10.14135/j.cnki.1006-3080.2017.02.006
Streptomyces sp.Tü 4128中新型抗生素bagremycins抗性基因bagJ的研究
Identification of bagJ as A Resistant Gene for Novel Antibiotic Bagremycins in Streptomyces sp.Tü 4128
摘要
Abstract
The function of gene bagJ via in-frame deletion in Streptomyces sp.Tü 4128 was identified.The result of HPLC analysis showed that the production of bagremycins was significantly decreased in the single knock-out strain of bagJ.The production of bagremycins was recovered in the complementation strain.The overexpression of gene bagJ resulted in producing far more bagremycins,which indicated that bagJ was indispensable in the biosynthesis of bagremycins.The test of inhibition zones showed that the bagJ mutant was more sentitive to bagremycins than the wild type.Heterogenous expression of BagJ in Streptomnyces lividans TK64 enhanced its endurance to bagremycins which suggested that bagJ might be a resistant gene of the novel antibiotics bagremycins.SEM showed that the morphology of the mycelia was changed in the heterogenous expression strain of BagJ in S.lividams TK64.The sensitivity assays of other antibiotics showed that BagJ was probably an antiporter.关键词
bagremycins/Streptomyces sp.Tü 4128/bagJ/MFS家族/抗性基因Key words
bagremycins/Streptomyces sp.Tü 4128/bagJ/MFS family/resistant gene分类
生物科学引用本文复制引用
张玉琛,吴海珍,鞠诚,祁双双,叶江,张惠展..Streptomyces sp.Tü 4128中新型抗生素bagremycins抗性基因bagJ的研究[J].华东理工大学学报（自然科学版）,2017,43(2):184-192,9.基金项目
国家自然科学基金(31200026) （31200026）
