河南农业大学学报2017，Vol.51Issue(2)：201-206,6.
猪细小病毒感染PK-15细胞后TLRs转录时相的变化
Changes of toll-like receptors transcriptional profiles of PK-15 cell cultures following infection with porcine parvovirus
摘要
Abstract
This study was conducted to detect the expression of TLRs after PPV infected PK-15 cell,which aimed to explove the theoretical basis of proven PPV infection mechanism.The transcription of TLR1-10 in PPV infected PK-15 cells was detected by real-time PCR method.The results showed that TLR1 in PK-15 cells after PPV infection up-regulated in 3 hours and was about 3.1 times of the control group cells,other times were lower than the normal level;TLR3 and TLR7 mRNA expression in the initial stage of infection were lower than the normal level;the content of mRNA TLR4 and TLR8 were 3,12 and 36 h after infection increased;TLR1O expression in 3 h and 36 h increased respectively,other times were reduced to varying degrees;while TLR2 and TLR9 expression level of mRNA in 24 h after infection began to increase,and reached the peak at 48 h,and respectively 10.1 times and 26.7 times higher than those in blank.Studies show that PPV infected PK-15 cells can induce the TLR2 and TLR9 in the mRNA level was significantly increased.PPV infection may be mediated by TLR2 and TLR9 receptors.关键词
猪细小病毒/Toll样受体/PK-15细胞/转录时相Key words
porcine parvovirus/Toll-loke receptor/PK-15 cell/transcriptional profiles分类
农业科技引用本文复制引用
周雍,靳晓慧,李炳晓,景亚星,韩丽,张利卫,魏战勇..猪细小病毒感染PK-15细胞后TLRs转录时相的变化[J].河南农业大学学报,2017,51(2):201-206,6.基金项目
国家自然科学基金项目(U140410985) （U140410985）
