河南农业大学学报2017，Vol.51Issue(2)：218-225,8.
菊花几丁质酶基因ChCHI的克隆及表达分析
Molecular cloning and expression analysis of a chitinase gene ChCHI from Chrysanthemum morifolium
摘要
Abstract
According to the conserved sequences of chitinase gene,the chitinase gene ChCHI was isolated from leaf of Chrysanthemum morifolium using RT-PCR and RACE method (The accession number in GenBank:KX881373).The full-length cDNA of CHCHI was 1385 bp,containing a 960 bp open reading frame which encodes 319 amino acids.Encoding product of ChCHI gene was a kind of hydrophilic and stable protein with a molecular weight of 33.5 kD and isoelectric point of 6.38.Prediction of secondary structure indicated that the protein was mainly composed of alpha helix and random coil.Sequence alignment and phylogenetic analysis showed that the amino acid sequence of ChCHI belongs to family 19 of glycoside hydrolase.CHCHI was highly identified with the chitinase protein from Mikania micrantha (ACO25187.1) with the similarity of 87％.The results of qRT-PCR analysis showed that the expression level of the ChCHI gene in leaves of Chrysanthemum morifolium was significantly increased under the SA treatments.The results implied that ChCHI gene may play an important role in the molecular regulation of resistance to environmental stress in chrysanthemum morifolium.关键词
菊花/几丁质酶基因/克隆/表达分析Key words
Chrysanthemum morifolium/chitinase gene/gene clone/expression analysis分类
农业科技引用本文复制引用
崔波,宋彩霞,王莹博,王若斓,蒋素华,梁芳,叶永忠..菊花几丁质酶基因ChCHI的克隆及表达分析[J].河南农业大学学报,2017,51(2):218-225,8.基金项目
郑州市重点科技攻关项目(141PZDGG189) （141PZDGG189）
