中医学报2017，Vol.32Issue(4)：486-489,4.DOI:10.16368/j.issn.1674-8999.2017.04.126
蛇床子素对人黑色素瘤A375细胞增殖和凋亡的影响
The Effect of Osthole in Proliferation and Apoptosis of Human Melanoma Cell Lines A375
摘要
Abstract
Objective:To investigate the effect of Osthole in proliferation and apoptosis of human melanoma cell lines A375.Methods:The human melanoma cell lines A375 were cultured in vitro and after the A375 cell at logarithmic growth phase was treated with different concentration of Osthole.The proliferation of A375 cell was detected by methyl thiazolyl tetrazolium (MTT) assay;the flow cytometry was used to determine the apoptosis of A375.RT-PCR was used to detect the mRNA level of Bcl-2 and Bax in A375;Western blotting was used to detect the expression of I-κB,p-NF-κB,NF-κB,Bcl-2 and Bax in human melanoma cell line A375.Results:Osthole can decrease the proliferation rate,the mRNA level of Bcl-2 and the expression of p-NF-κB and Bcl-2 protein level in human melanoma cell line A375 in a concentration dependent manner.Moreover,Osthole can increase the apoptosis of A375 cells and the mRNA level of Bax and the expression of IκB-α and Bax protein level and had no influence in the expression of NF-κB.Conclusion:Osthole could induce the proliferation inhibition and apoptosis of A375 cell and the effect is associated with suppressing NF-κB signal transduction pathway activation.关键词
黑色素瘤/蛇床子素/A375细胞/细胞增殖/细胞凋亡/NF-κB信号通路/肿瘤/中医药治疗Key words
melanoma/Osthole/A375 cell/proliferation/apoptosis/NF-κB signal pathway/Tumor/TCM therapy分类
医药卫生引用本文复制引用
卢娟,陈赵慧,杨今言..蛇床子素对人黑色素瘤A375细胞增殖和凋亡的影响[J].中医学报,2017,32(4):486-489,4.基金项目
新疆医科大学创新基金(XJC201257,XYDCX201531) （XJC201257,XYDCX201531）
