黑龙江农业科学Issue(1)：20-23,4.DOI:10.11942/j.issn1002-2767.2017.01.0020
不同培养条件对菜用大黄组培苗玻璃化的影响
Effects of Different Conditions on Tissue Culture Vitrification of Rheum rhaponticum L.
摘要
Abstract
In order to prevent vitrification seedling of Rheum rhaponticum L.,and improve the quality of somaclone,taking Rheum rhaponticum L.buds as explants,with 1/2MS as the basic culture medium,the effects of sucrose,agar and 6-BA on the vitrification of tissue cultured seedlings were studied.Meanwhile,physiological and biochemical indexes between vitrification shoots and normal seedlings were determined and compared.The results showed that the high coefficient of proliferation and the low rate of vitrification was cultured in 1/2 MS medium supplemented with 30 g·L-1 sucrose,6 g·L-1 agar,1.0 mg·L-1 6-BA and 1.0 mg·L-1 NAA.The soluble sugar and soluble protein content of the normal seedlings was significantly higher than that of the vitrification shoots,while the water content of the tissue was slightly higher than that of the normal seedlings.关键词
菜用大黄/组织培养/玻璃化Key words
Rheum rhaponticum L./conditions of tissue culture/vitrification分类
农业科技引用本文复制引用
牛延斌,邵珠田,高二娟,姜立娜..不同培养条件对菜用大黄组培苗玻璃化的影响[J].黑龙江农业科学,2017,(1):20-23,4.基金项目
河南省科技厅基础前沿资助项目(122300410134) （122300410134）
河南科技学院人才引进资助项目(207010612015) （207010612015）
国家级大学生创新训练资助项目(2015CX044) （2015CX044）
