黑龙江农业科学Issue(3)：138-143,6.DOI:10.11942/j.issn1002-2767.2017.03.0138
激光扫描共聚焦显微镜在植物研究中的应用
Application of Laser Scanning Confocal Microscopy in Plant Research
摘要
Abstract
Fluorescence imaging technology,optical microscope and computer image analysis system spawned a perfect combination of laser scanning confocal microscope(LSCM) ,and its superior structure features to achieve the thicker sample could be scanned from point to layer into a clear image,which was conducive to all kinds of fluorescence signal acquisition,three-dimensional reconstruction and quantitative analysis of sample the multiplex fluorescence signal acquisition.These advantages greatly enhanced the ability to explore the basic functions of biological space-time expression from the cellular and molecular levels.Laser scanning confocal microscopy were reviewed in the fields of plant tissue chemistry,plant organelles and cytoskeleton,plant development and so on,with the increasing diversification of the technology,.关键词
激光扫描共聚焦显微镜/细胞骨架/Ca2+/荧光染料Key words
laser scanning confocal microscope(LSCM)/cell skeletons/Ca2+/fluorescent dye分类
生物科学引用本文复制引用
李珊珊,刘松,宋克难,邹运,张珍珠..激光扫描共聚焦显微镜在植物研究中的应用[J].黑龙江农业科学,2017,(3):138-143,6.基金项目
国家级大学生创业创新训练计划资助项目(201510221012) （201510221012）
黑龙江省教育厅基本科研业务专项资助项目(齐齐哈尔大学科学研究类项目)(135109255) （齐齐哈尔大学科学研究类项目）
齐齐哈尔市科学技术计划资助项目(NYGG-201518) （NYGG-201518）
齐齐哈尔大学青年教师科研支持计划资助项目(2014k-M22) （2014k-M22）
