核农学报2017，Vol.31Issue(4)：627-634,8.DOI:10.11869/j.issn.100-8551.2017.04.0627
盐胁迫诱导玉米苗期DNA甲基化变异的研究
Analysis of the DNA Methylation of Maize in Response to Salt Stress
摘要
Abstract
To determine the changes of DNA methylation under different concentrations of salt stress,the leaves of maize at the seedling stage were used to estimate the levels and patterns of cytosine methylation by methylation sensitive amplified polymorphism (MSAP) method.The results showed that the DNA methylation ratio is 52.79％,57.47％,51.98％,47.39％,49.16％,47.06％ under 0 (control),50,100,150,200,250 mmol·L-1 NaCl stress,respectively.However,only 50 mmol· L-1 NaCl treatment showed higher DNA methylation ratio than in the control,and at other concentrations,the DNA methylation ratios were declining as concentrations increased but lower than control,which indicate that the de-methylation might play a significant role in salt stress (1.8％ to 12.2％).In addition,sequencing and blast results showed that a total of 16 methylation sites were detected,which may relate to many genes of plant growth and development.These experimental results provided a theoretical basis for further study on the salt resistant mechanism of maize.关键词
玉米/盐胁迫/苗期/DNA甲基化/变异Key words
maize/salt stress/seedling/DNA methylation/variation引用本文复制引用
孙丽芳,胡凯凤,高树仁,王霞,赵伟,苗兴芬,杨克军..盐胁迫诱导玉米苗期DNA甲基化变异的研究[J].核农学报,2017,31(4):627-634,8.基金项目
黑龙江省青年科学基金(QC2015029),农业部公益性项目(201303007) （QC2015029）
