核农学报2017，Vol.31Issue(4)：643-653,11.DOI:10.11869/j.issn.100-8551.2017.04.0643
紫色黄秋葵转录组功能基因测序及分析
The Purple Abelmoschus esculentus Transcriptome as a Source for Gene Sequence Information
摘要
Abstract
Okra,belonging to a group of edible and medical plants,is rich in various bioactive substances such as anthocyanin,polysaccharide,flavoniod,diterpenoid,etc.In order to explore genetic basis for the synthesis of these active substances,the transcriptome of purple Abelmoschus esculentus was sequenced by Illumina HiSeq 2500 platform which is a new generation of high-throughput sequencing technology.A total of 23 026 500 reads fragment were generated by sequencing.The reads gave 42 484 Unigenes with an average length of 877 bp.31 931 and 21 926Unigenes were annotated against the Nr and SwissProt databases respectively.According to COG and KOG databases,the Unigenes were further annotated and grouped into 24 and 25 functional categories,respectively.20 031 Unigenes were annotated against Pfam database and formed 7 277 protein structural domains.18 602 Unigenes annotated against GO database were divided into cellular component,molecular function and biological process categories with a total of 51 functional groups.Many of 18 602 Unigenes were closely related to cell part,cell,catalytic activity,binding metabolic process and cellular process.KEGG pathway analysis showed that 7 619 Unigenes could be broadly classfied into 119 classes according to their function,and some of them were involved in anthocyanin biosynthesis (1 Unigene),flavone and flavonol biosynthesis (3),flavonoid biosynthesis (35),N-glycan biosynthesis (61),diterpenoid biosynthesis (13)and terpenoid backbone biosynthesis (70).The Unigenes sequenced and functional annotations would lay a provide the foundation for follow-up study of synthetic ways and related genes of the bioactive substances.关键词
紫色黄秋葵/转录组基因/测序分析/功能注释Key words
purple Abelmoschus esculentus/transcriptome/sequencing/function annotation引用本文复制引用
张少平,邱珊莲,郑云云,张帅,吴松海,何炎森,郑开斌..紫色黄秋葵转录组功能基因测序及分析[J].核农学报,2017,31(4):643-653,11.基金项目
福建省公益类科研院所专项(2016R1012-4),福建省农业科学院青年英才计划项目(YC2015-19),福建省农业科学院科技创新团队PI项目(2016PI-2) （2016R1012-4）
