核农学报2017，Vol.31Issue(4)：654-662,9.DOI:10.11869/j.issn.100-8551.2017.04.0654
LRR-RLKs亚家族基因RLK6在拟南芥开花过程中的作用
Study of RLK6, One Member of Leucine-rich Repeat Receptor-like Kinases (LRR-RLKs) Subfamily Genes, on Process of Flowering in Arabidopsis
摘要
Abstract
To investigate the functions of LRR-RLKs gene RLK6 in Arabidopsis,a pSuper1300-RLK6 expression vector was constructed and five RLK6 overexpression transgenic lines were obtained.The two lines,OE8 and OE9,were selected for further analysis.The homozygous RLK6 mutants (rlk6-2,rlk6-3 and rlk6-6),OE8,OE9 and WT were characterized for phenotype analysis,and the result showed that RLK6 mutants appeared to show similar phenotype with wild-type (WT) during growth and development.But,OE lines displayed obviously early flowering than WT.Four key genes which relates to flowering were examined in all genotypes by using qRT-PCR.In OE lines,increased expression of floral activator genes (CO,SOC1 and LFY) with the decreased level of floral repressor gene (FLC) was observed,which was consistent with it phenotypes,when compared to WT.These results suggest that RLK6 play a key role in promoting flowering in Arabidopsis,and these results will lay the foundation for understanding the mechanisms of RLK6.关键词
拟南芥/LRR-RLKs/过量表达/开花Key words
Arabidopsis/LRR-RLKs/overexpression/flowering引用本文复制引用
杨敏,韩玉珍,阿依江·哈拜克,王翠玲..LRR-RLKs亚家族基因RLK6在拟南芥开花过程中的作用[J].核农学报,2017,31(4):654-662,9.基金项目
国家自然科学基金(31570254),博士科研启动基金(09001770) （31570254）
