核农学报2017，Vol.31Issue(4)：671-679,9.DOI:10.11869/j.issn.100-8551.2017.04.0671
擎天凤梨SAMs基因的分离及开花期的表达分析
Isolation of Guzmania SAMs Genes and Expression in Flowering Period
摘要
Abstract
In order to obtain guzmania SAMs(S-adenosylmethionine synthetase) genes sequence and know their function in ethylene biosynthesis pathway,based on full-length cDNA library constructed and batch EST sequencing in the early stage,we performed Primer Walking Sequencing to obtain two SAMs genes,named SAMsl and SAMs2,respectively.SAMsl (GenBank:KF787113) cDNA is 1 375bp in length,and has a 1 086 bp open reading frame that encode 361AA amino acid residuals.SAMs2(GenBank:KF787114) is 1 235bp and has a 945 bp open reading frame that encode 314AA amino acid residuals.Their amino acid composition,physical and chemical properties,conserved domains,secondary structure,and tertiary crystal structure and had been predicted and analyzed,by bioinformatics methods.Molecular phylogenetic analysis showed that SAMsl were clustered with SAMs gene of grass family into one group,while far from SAMs2.That analysis of gene expression of the two genes and ethylene release production among different tissues in flowering stage showed SAMsl’s gene expression change trend consistent with ethylene release trend.Namely,those of red bracts were the highest,followed by green bracts,finally green leaves.Therefore,we considered that SAMsl may play an important role in ethylene biosynthesis pathway.SAMs2 may be involved in other S-adenosylmethionine biological metabolic processes in addition to ethylene biosynthesis.The study had an important sense in ethylene biosynthesis,bract coloration mechanism and flower induction in Guzmania.关键词
擎天凤梨/SAMs 基因/克隆/乙烯Key words
Guzmania Ruiz & Pav/S-adenosylmethionine synthetase/cloning/ethylene引用本文复制引用
刘建新,丁华侨,郁永明,田丹青,王炜勇..擎天凤梨SAMs基因的分离及开花期的表达分析[J].核农学报,2017,31(4):671-679,9.基金项目
浙江省自然科学基金(LQ13C150002),浙江省公益性技术应用研究计划项目(2012C22085) （LQ13C150002）
