中医药导报2017，Vol.23Issue(7)：77-79,3.
HPLC-ELSD法测定特制接骨丸中三七皂苷R1、人参皂苷Rg1、Re的含量
Determination of Notoginsenoside R1, Ginsenosides Rg1 and Re in Tezhi Jiegu Pills（特制接骨丸）By HPLC-ELSD
杜志谦 1张利静 2夏华玲 2马守芳 1郑超2
作者信息
- 1. 河南省正骨研究院,河南洛阳471000
- 2. 河南中医药大学,河南郑州450008
- 折叠
摘要
Abstract
Objective:To establish a determination of the content of Tezhi Jiegu Pills notoginsenoside R1,ginsenosides Rg1and ginsenosides Re by HPLC-ELSD.Methods:HPLC-ELSD was used.The analysis was carried on WondaSil C1s ODS (4.6 mm×250 mm,5 μm) column;the mobile phase was the acetonitrile (A)-water (B);the gradient elution (0-10min,19％→21％ A;10-19min,21％→23％ A;19min-20min,23％→32％ A;20-28 min,32％ A;28-29 min,32％→33％ A;29-40min,33％ A;40-41 min,33％→19％ A;41-48 min,19％ A);the column temperature was 25 ℃;The flow rate was 1.0mL·min-1;The drift tube temperature was 108 ℃;the gas flow was 2.8 L·min-1.Results:Notoginsenoside R1,ginsenosides Rg1and ginsenosides Re respectively in 0.515-1.030,2.500-5.000 and 1.510-3.020 μg good linearity in the range.The average recovery and RSD of notoginsenoside R1,ginsenosides Rg1and ginsenosides Re respectively were 99.67％,97.49％ and 101.74％.Conclusion:HPLC-ELSD method can accurately and quickly realize the quantitative determination of the saponins in the s Tezhi Jiegu Pills,and it can be used for control the quality.关键词
特制接骨丸/三七皂苷R1/人参皂苷Rg1、人参皂苷Re/含量测定Key words
Tezhi Jiegu Pills/Notoginsenoside R1/Ginsenosides Rg1/Ginsenosides Re/content determination分类
医药卫生引用本文复制引用
杜志谦,张利静,夏华玲,马守芳,郑超..HPLC-ELSD法测定特制接骨丸中三七皂苷R1、人参皂苷Rg1、Re的含量[J].中医药导报,2017,23(7):77-79,3.
