海洋科学2017，Vol.41Issue(1)：76-82,7.DOI:10.11759/hykx20160423003
绿潮藻浒苔微生物高效降解及生物乙醇制备
Screening and characterization of a newly isolated strain for microbiological degradation of Ulva prolifera and bioethanol production
摘要
Abstract
Ulva proliferahas been the dominant species in the huge annual biomass accumulation during green algae bloom in Yellow Sea since 2007. This species can be used for energy utilization by fermenta-tion under certain conditions. In this study, we identified ahighlyefficient cellulose-decomposing micro-organismbyscreeningthe rotten algae and employedit as a raw material to manufacture bioethanol. We studied its cellulose activity and the ability to decomposeU.prolifera atdifferentreaction temperatures, times.pH, and dosages of crude enzyme. We identified the strain with highest efficiency in cellu-lose-decomposition using Congo red staining method and ITSand termed it as theF strain (34.79U/mL). We also foundthatthe optimum conditionsfor the fermentation of U. prolifera were as follows: crude en-zyme, 6%; treating temperature, 38℃; treating time, 60 h; and pH, 6.8. Under these optimum conditions-bioethanol yield of up to 28.98g/Lwas achieved.关键词
浒苔(Ulva prolifera)/纤维素降解菌/发酵/生物乙醇Key words
Ulva prolifera/cellulose-decomposing microorganisms/fermentation/bioethanol分类
海洋科学引用本文复制引用
王淑贤,韦章良,贾睿,张建恒,霍元子,于克锋,何培民..绿潮藻浒苔微生物高效降解及生物乙醇制备[J].海洋科学,2017,41(1):76-82,7.基金项目
国家自然科学基金(41576163) （41576163）
国家重点研发计划项目(2016YFC1402105) （2016YFC1402105）
国家海洋局公益性科研专项(201205010, 201105023) （201205010, 201105023）
科技支撑计划项目(2012BAC07B023)Nation Natural Science Foundation of China, No.41576163 （2012BAC07B023）
The National Key Research and Development Plan, No.2016YFC1402105 （）
Public Science and Technology Research Funds Projects of the Ocean, No.201205010, No.201105023 （）
National Science & Technology Pillar Program, No.2012BAC07B03 （）
