基础医学与临床2017，Vol.37Issue(5)：643-647,5.
纺锤菌素抑制胃癌细胞的侵袭和转移
Netropsin inhibits invasion and migration ability of gastric cancer cells
摘要
Abstract
Objective To investigate the effect of netropsin on migration and invasion ability of gastric cancer cells and its mechanisms.Methods To determine if netropsin inhibits migration and invasion of gastric cancer cells, Transwell migration and invasion assay was performed.Then Western blot was performed to detect expression of E-cadherin and vimentin in gastric cancer cells with or without presence in medium netropsin.Finally, immunofluorescence was performed to detect changes in the cellular localization of β-catenin to validate whether Wnt/β-catenin pathway was suppressed or not.Results Netropsin with the concentration of 25 μmol/L had minimal inhibition effect on cell proliferation and was able to suppress ability of migration and invasion by inhibiting EMT in gastric cancer cells(P<0.05).Meanwhile netropsin was able to down-regulate the expression of epithelial markers E-cadherin and up-regulate the expression of mesenchymal marker vimentin.Finally,immunofluorescence showed that netropsin was able to block translocation of β-catenin from cytoplasm to nuclear.Conclusions Netropsin can inhibit EMT thereby suppressing migration and invasion of gastric cancer cells.The mechanism is that netropsin can compete with HMGA2 for transcription factor binding site thereby suppressing the Wnt/β-catenin pathway.关键词
胃肿瘤/上皮间质转换/纺锤菌素Key words
gastric carcinoma/epithelial-mesenchymal transitions(EMT)/netropsin分类
医药卫生引用本文复制引用
查郎,彭旭东,王子卫..纺锤菌素抑制胃癌细胞的侵袭和转移[J].基础医学与临床,2017,37(5):643-647,5.基金项目
国家自然科学基金(81272753) （81272753）
