基础医学与临床2017，Vol.37Issue(5)：653-657,5.
β淀粉样蛋白及代谢酶类在阿尔茨海默病和糖尿病模型小鼠脑内的表达
Expressions of amyloid beta peptide and its related metabolic enzymesin the brain of model mice with Alzheimer`s disease and type 2 diabetes mellitus
摘要
Abstract
Objective To examine the changes of Aβ expression and its related metabolic enzymes in the brains of AD and T2DM mice, so as to explore the possible mechanism of type 2 diabetes mellitus combined with AD.Methods Five-month-old APP/PS1 double transgenic mice, ob/ob mice and the wild-type control mice were employed in this study.Immunohistochemical (IHC) staining, Elisa and Western blot were used to detect SP, Aβ and its related metabolic enzymes.Results A certain number of SPs were observed in the cerebral cortex and hip-pocampus of APP/PS1 mice;SPs were occasionally observed in the cortex of ob/ob mice, while no SP appeared in wild-type mice.Aβ40 and Aβ42 levels were significantly increased in APP/PS1 and ob/ob mice brains as compared with controls (P<0.05), thought both Aβ40 and Aβ42 levels in AD mice were significantly higher than those of ob/ob mice (P<0.05).APP expression level was highest in APP/PS1 mice among 3 groups, and its expressed higher in ob/ob mice than that of control mice (P<0.05).BACE1 expression was notably increased in APP/PS1 and ob/ob mice as compared with control(P<0.05), however, it expressed higher in APP/PS1 mice than ob/ob mice (P<0.05).The expression of Aβ degradation enzyme IDE was reduced in APP/PS1 and ob/ob mice(P<0.05), while lowest in ob/ob mice.Conclusions Overexpression of Aβ may be one of main reasons for T2DM combined AD.关键词
β淀粉样蛋白/老年斑/阿尔茨海默病/2型糖尿病Key words
Aβ/senile plaques/Alzheimer`s disease/type 2 diabetes mellitus分类
医药卫生引用本文复制引用
刘滢,贺桂琼,覃万翔,罗敏,汪克建..β淀粉样蛋白及代谢酶类在阿尔茨海默病和糖尿病模型小鼠脑内的表达[J].基础医学与临床,2017,37(5):653-657,5.基金项目
国家自然科学基金(81371221) （81371221）
重庆市基础与前沿研究计划项目(cstc2016jcyjA0069) （cstc2016jcyjA0069）
重庆市教委科学技术研究项目(KJ130317) （KJ130317）
重庆市渝中区科技项目(20130132) （20130132）
