基础医学与临床2017，Vol.37Issue(5)：668-675,8.
IL-1β通过JAK2-STAT3促进大鼠脊髓损伤后胶质瘢痕形成
IL-1β promotes glial scar formation after spinal cord injury in rats by JAK2-STAT3
摘要
Abstract
Objective To investigate the mechanism of IL-1β in promoting glial scar formation after spinal cord injury.Methods The experimental model of SCI was created by extradural compression of the spinal cord using an aneurysm clip.Rats were randomly divided into model group, sham operation group, IL-1β inhibitor IL-1RA group, IL-1β group and IL-1β+JAK2-STAT3 inhibitor AG490 group, according to different interventions, then were given normal saline, IL-1RA, IL-1β and IL-1β+AG490 every 10 μL respectively, sham group received only laminectomy.The motion function of the hindlimbs of rats was measured by Basso Beattie Bresnahan(BBB) scores and the expression of GFAP, vimentin and p-STAT3 were detected by Western blot technique, immunofluorescence assay and immunohistochemistry technique at corresponding time points(at the 8th, 12th hour, 1st, 3rd, 7th and 14th day after SCI).Results The expression trend of p-STAT3(at the 8th and 12th hour after SCI),GFAP and vimentin(at the 7th and 14th day after SCI)was: the expressions of p-STAT3, GFAP and vimentin in the model group were significantly higher compared with the sham group(P<0.01), the expression of p-STAT3,GFAP andvimentin in the IL-1RA group were significantly lower compared with the model group(P<0.05) whereas significantly higher compared with the sham group(P<0.05);the expressions of p-STAT3, GFAP and vimentin in the IL-1β+AG490 group were significantly lower compared with the model group(P<0.05)whereas significantly higher compared with the sham group(P<0.05), the expressions of p-STAT3, GFAP and vimentin in the IL-1β group were significantly higher compared with the model group(P<0.05).Conclusions IL-1β can improve glial scar formation via JAK2-STAT3 signal.Inhibition of IL-1β or JAK2-STAT3 can reduce glial scar formation and promote functional recovery of spinal nerve.关键词
脊髓损伤/IL-1β/JAK2-STAT3/GFAP/vimentinKey words
spinal cord injury/IL-1β/JAK2-STAT3/GFAP/vimentin分类
医药卫生引用本文复制引用
刘敬贤,夏永智,王富贵,唐维,晏怡..IL-1β通过JAK2-STAT3促进大鼠脊髓损伤后胶质瘢痕形成[J].基础医学与临床,2017,37(5):668-675,8.基金项目
国家自然科学基金(81100906) （81100906）
重庆市自然科学基金(cstc2012jjA10055) （cstc2012jjA10055）
国家临床重点专科建设项目经费([2011]170) （[2011]170）
