解剖学杂志2017，Vol.40Issue(2)：132-136,5.DOI:10.3969/j.issn.1001-1633.2017.02.003
西格列汀对Ⅱ型糖尿病大鼠骨髓内皮祖细胞衰老的影响及可能机制
Effect of sitagliptin on senescence of endothelial progenitor cells of bone marrow in type 2 diabetes mellitus rats and its possible mechanism
摘要
Abstract
Objective:To study the effect of sitagliptin on senescence of endothelial progenitor cells (EPCs) of bone marrow in type 2 diabetes mellitus rat and its possible mechanism.Methods:Type 2 diabetes mellitus rat model was induced by high amounts of sugar and fat chow combined with single injection of streptozotocin (35 mg · kg-1).After the model was established,type 2 diabetes mellitus rats were randomly divided into model (DM)group,seitagliptin injection group and seitagliptin combined with nicotinamide injection (seitagliptin+NAM) group.Normal rats were randomly divided into normal control (NC) group and nicotinamide injection (NAM) group.The weight and blood glucose of rats in all groups were detected each week.The DM and NC groups were received physiologic saline administration and the treatment groups with seitagliptin or NAM for 4 weeks.The level of dipeptidyl deptidase-4 (DPP-4) and glucagon like peptide-1 (GLP-1) in plasma were measured by ELISA,the percentage of senescenced EPCs from bone marrow and the mRNA expression of SIRT1 were measured by RT qPCR.Results:Compared with the NC group,the percentage of senescenced EPCs in the NAM group reduced significantly (P＜0.05).The level of DPP-4 and GLP-1 in plasma and the mRNA expression of SIRT1 showed no significant difference between the NC group and NAM group.Compared with the NC group,the level of DPP-4 in plasma and the percentage of senescenced EPCs from bone marrow increased.The level of GLP-1 in plasma and the mRNA expression of SIRT1 in EPCs decreased in DM group,and showed significant difference between the NC and DM group.Compared with the DM group,the level of DPP-4 in plasma and the percentage of senescenced EPCs from bone marrow decreased.The level of GLP-1 in plasma and the mRNA expression of SIRT1 in EPCs increased in seitagliptin group in a dose-dependent manner,which had a significant difference between the DM and seitagliptin group.Compared with the sitagliptin (30 mg · kg-1) group,the percentage of senescenced EPCs from bone marrow increased in sitagliptin+NAM group,which had a significant difference between the sitagliptin and sitagliptin+ NAM group.Conclusion:Seitagliptin reduces senescence of EPCs in type 2 diabetic rats which may involve in the SIRT1/DPP-4/GLP-1 pathway.关键词
内皮祖细胞/西格列汀/烟酰胺/沉默信息调节因子1/二肽基肽酶4/胰高血糖素样肽-1/Ⅱ型糖尿病/大鼠Key words
endothelial progenitor cell/seitagliptin/nicotinamide/SIRT1/dipeptidyl deptidase-4/glucagon like peptide-1/type 2-diabetes mellitus/rat引用本文复制引用
庹磊,袁琼,周乾毅,赵攀,徐洁,杨羚,谢芬,金晓霞,周洋,王传杰,周英..西格列汀对Ⅱ型糖尿病大鼠骨髓内皮祖细胞衰老的影响及可能机制[J].解剖学杂志,2017,40(2):132-136,5.基金项目
湖北省大学生创新项目(20141088014) （20141088014）
