解剖学杂志2017，Vol.40Issue(2)：145-148,156,5.DOI:10.3969/j.issn.1001-1633.2017.02.006
老年大鼠后肢高血流剪切力诱导的侧支血管生长及其与血管生成素-1表达的关系
Collateral growth induced by high fluid shear stress in the hindlimbs of aged rats and its relationship with the expression of angiopoietin-1
郭宜竞 1邱要颜 1蔡维君2
作者信息
- 1. 中南大学湘雅医学院,长沙410013
- 2. 中南大学基础医学院组织学与胚胎学教研室,长沙410013
- 折叠
摘要
Abstract
Objective:To detect the collateral growth induced by high fluid shear stress in the hindlimbs of aged rats and its relationship with the expression of angiopoietin-1 (Ang-1).Methods:Twelve healthy aged rats were chosen as the study group,where the left femoral arteries and veins of rats were separated and the right femoral arteries and veins were anastomosed.One week and four weeks later,six of them were sacrificed each time respectively.The collateral growth was detected by angiography and the expression of Ang-1 was detected by confocal immunofluorescence.Three other aged rats were used as normal controls.Results:The collateral growth of the control group and sham-operation group showed no significant difference.The collateral growth of anastomosis group was significantly increased,which included the significant difference between one-week group and four-week group.The Ang-1 expression of the control group and sham-operation group showed no significant difference,which was very weak and only appeared in tunica media and parts of adventitia.Oneweek anastomosis group showed more Ang-1 expression not only in the same area but also in the endotheliurn.And four-week anastomosis group showed highly positive expression of Ang-1.In the anastomosis group,the expression of Ang-1 was significantly increased.Besides,four-week anastomosis group also showed significant increase compared to one-week anastomosis group.Conclusion:Collateral growth can be induced by high fluid shear stress in the hindlimbs of aged rats,which is positively related to the increased expression of Ang-1 in the related area.关键词
高血流剪切力/侧支血管生长/血管生成素-1/老年大鼠Key words
high fluid shear stress/collateral growth/angiopoietin-1/aged rat引用本文复制引用
郭宜竞,邱要颜,蔡维君..老年大鼠后肢高血流剪切力诱导的侧支血管生长及其与血管生成素-1表达的关系[J].解剖学杂志,2017,40(2):145-148,156,5.
