军事医学2017，Vol.41Issue(4)：260-264,286,6.DOI:10.7644/j.issn.1674-9960.2017.04.003
查尔酮类化合物L2H17对甲型流感病毒FM1株感染小鼠的保护作用
Protective effect of chalcone ketones compound L2H17 on mice infected with influenza virus
摘要
Abstract
Objective To investigate the protective and therapeutic effect of chalcone ketones compound(code:L2H17,hereinafter referred to as L2)on mice infected with influenza A virus.Methods BALB/c mice were randomly divided into six groups:normal group,model group,positive drug-treated group,L2 treated groups (3 different concentrations).The mice were adapted for 72 hours,before a model was established by intranasal infection.Mice in each group were given medicine by i.g once daily for 6 days starting 24 h before virus challenge.Survival was observed daily for 14 days.The mortality,median survival time,rate of death protection and rate of prolonging life were determined to observe the therapeutic effect of chalcone(L2) against influenza virus infection.The whole lungs were taken under aseptic conditions on days 3 and 5 post-infection to calculat lung indexes and lung index inhibition.The left lung was fixed with 4% formaldehyde for pathological biopsy,the right lung was soaked in RNAstore to detect lung tissue viral load,and the double antibody sandwich ELISA method was used to detect the expression of inflammatory cytokines IL-6 and TNF-α in order to observe the therapeutic effect of L2 on viral pneumonia caused by influenza virus infection.Results Compared with the model group,the L2 80 mg/kg treatment group exhibited significant increases in median survival time(11 d),the rate of death protection (50%),and the rate of prolonging life(24.1%)but a moderate 50% decrease in mortality.In addition,the lung index decreased significantly both on d 3 and 5 after virus infection (P<0.05).The pathological results also improved significantly.The L2 80 mg/kg dose group had a significantly lower viral load of lung tissue on d 3 and 5 post-infection(P<0.05).Compared with model group,the expression of inflammatory factor IL-6 became lower to different degrees.Conclusion L2 has a protective effect on mice infected with influenza virus by reducing the degree of pathological changes of pneumonia caused by influenza viruses.关键词
流感病毒A型/病毒性肺炎/查尔酮类化合物/肿瘤坏死因子α/白细胞介素6Key words
influenza A virus/viral pneumonia/chalcone ketones compound/TNF-α/IL-6分类
医药卫生引用本文复制引用
史鹏辉,李剑波,梁广,张亚利,王晓龙,杨静,王升启..查尔酮类化合物L2H17对甲型流感病毒FM1株感染小鼠的保护作用[J].军事医学,2017,41(4):260-264,286,6.基金项目
国家自然科学基金重点资助项目(81230089) （81230089）
国家自然科学基金面上项目(81473184,31270197) （81473184,31270197）
