军事医学2017，Vol.41Issue(4)：269-272,277,5.DOI:10.7644/j.issn.1674-9960.2017.04.005
高压水动力法建立肝特异性白细胞介素-6报告基因小鼠模型
Establishment of liver IL-6 reporter mouse model by hydrodynamic gene transfection
摘要
Abstract
Objective To construct a mouse model for real-time,noninvasive and specific monitoring of inflammation activation in hepatic tissues.Methods An inflammation reporter gene was targeted to the liver by hydrodynamic gene delivery technology.Bioluminescence imaging was used to detect the firefly luciferase(Fluc) expression in the mouse liver after inflammatory stimulation.Besides,the relevance between the light intensity and inflammation level was also intensively investigated.Results pIL-6-Fluc was successfully delivered to the liver.The hydrodynamic gene delivery could cause a transient liver injury that could return normal in 5 to 7 days.The expression of pIL-6-Fluc could be induced by lipopolysaccharides(LPS) treatment with an about (46.80±13.35) fold increase at the peak value,which was significantly higher than that detected by ELISA [(4.09±0.96)fold].Conclusion An inflammation reporter mouse model is constructed in this study by hydrodynamic gene transfection,allowing noninvasive monitoring of inflammation activation specifically in hepatic tissues.The reporter model is capable of monitoring inflammation activation with a sensitivity higher than that of ELISA.关键词
基因转染/白细胞介素-6/活体荧光成像/肝脏炎症/小鼠,转基因Key words
gene transfection/interleukin-6/bioluminescence imaging/liver inflammation/mice/transgenic分类
生物科学引用本文复制引用
马聪,张玉龙,周欠欠,赵嫚,王小慧,江新泉,詹林盛..高压水动力法建立肝特异性白细胞介素-6报告基因小鼠模型[J].军事医学,2017,41(4):269-272,277,5.基金项目
国家自然科学基金面上资助项目(81371806) （81371806）
国家自然科学基金资助项目(81401369) （81401369）
军队"十三五"重点项目(BWS16J006-08) （BWS16J006-08）
