军事医学2017，Vol.41Issue(4)：273-277,5.DOI:10.7644/j.issn.1674-9960.2017.04.006
高原低氧加重脂多糖诱导大鼠肺组织的慢性炎症
High altitude hypoxia aggravates chronic inflammation induced by LPS in rats
摘要
Abstract
Objective To investigate the effect of high altitude hypoxia on chronic inflammation in rats.Methods Forty SD rats were randomly divided into 4 groups: control group (Con),chronic inflammation group (CI),high altitude hypoxia group (HH),high altitude hypoxia+chronic inflammation group (HH+CI).Rats in CI group were injected with lipopolysaccharide (LPS) (0.5 mg/kg) through the caudal vein twice a week for 4 weeks.Rats in HH+CI group were treated just as CI group was,but together with HH group rats were settled in a hypoxic environment of 6000 m altitude for three days.Pathological changes in lung tissues were observed by hematoxylin eosin stain.The peripheral white blood cell count and classification were measured.The levels of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in serum and lung tissues were detected by enzyme-linked immunosorbent assay (ELISA).Changes in IL-6 expression in rat lung tissues were observed by Western blotting.Results After LPS and high altitude hypoxia exposure,inflammatory cells infiltration and alveolar capillary expansion were observed in rats' lung tissue.Compared with Con group,not only the peripheral white blood cell count,but also the level of IL-6 and TNF-α in serum and lung tissue increased in CI and HH group(P<0.01).IL-6 expression levels observed by Western blotting were also increased in HH and CI group(P<0.01).High altitude hypoxia and chronic inflammation interacted(P<0.01).The peripheral white blood cell count was higher in HH+CI group than in other groups,and IL-6 and TNF-α expressions in lung tissue were increased(P<0.05).Conclusion An LPS-induced chronic inflammation model in rats is successfully obtained,and high altitude hypoxia could aggravate chronic inflammation.关键词
炎症/高原低氧/脂多糖类/白细胞介素6/肿瘤坏死因子αKey words
inflammation/high altitude hypoxia/lipopolysaccharides/interleukin-6/tumor necrosis factor-alpha分类
医药卫生引用本文复制引用
张益,李光宗,郁硕,陈锋,刘英富,霍景瑞,郑淑芳..高原低氧加重脂多糖诱导大鼠肺组织的慢性炎症[J].军事医学,2017,41(4):273-277,5.基金项目
天津市科技计划资助项目(14ZCDZSY00033) （14ZCDZSY00033）
武警后勤学院附属医院种子基金资助项目(FYM201514) （FYM201514）
武警后勤学院附属医院科研平台开放基金资助项目(WYKFZ201603,WYKFM201602) （WYKFZ201603,WYKFM201602）
全军重点实验室开放基金资助项目(JY1402) （JY1402）
