检验医学与临床2017，Vol.14Issue(9)：1220-1223,4.DOI:10.3969/j.issn.1672-9455.2017.09.007
基因芯片技术在检测痰涂阳肺结核患者结核分枝杆菌耐药性的应用效果
Evaluation of genechip in rapid detection of patients with multidrug-resistant tuberculosis
摘要
Abstract
Objective To analyze the performance of genechip in rapid detecting multidrug-resistant tuberculosis (MDR-TB).Methods The sputum samples collected from 1 030 smear positive TB patients detected in five districts of Chongqing were analyzed by genechip for detecting INH-and RFP-resistance.Simultaneously,the sputum samples were cultured with Lwenstein-Jensen culture medium followed by conventional drug susceptibility testing (DST) for detecting INH-and RFP-resistance.The conventional DST results by proportion method were served as the gold standard to evaluate the detection capability of genechip.Results Finally 958 patients with the results of genechip and DST were analyzed.The coincidence rate,sensitivity,specificity,positive predictive value and negative predictive value of genechip for INH drug resistance were 94.1%,80.2%,95.9%,71.8% and 97.4%,respectively.Compared with conventional DST,the difference had no statistical significance (P>0.05).And Kappa value was 0.724.The coincidence rate,sensitivity,specificity,positive predictive value and negative predictive value of genechip for INH drug resistance were 96.3%,86.4%,97.5%,80.9% and 98.3%,respectively.Compared with conventional DST,the difference had no statistical significance(P>0.05).And Kappa value was 0.815.Additionally,coincidence rate,sensitivity,specificity,positive predictive value and negative predictive value of NH-and RFP-resistance were 96.9%,78.3%,98.3%,78.3% and 98.3%,and Kappa value was 0.766.Compared with conventional DST,the difference had no statistical significance(P>0.05).Conclusion Genechip can be used for early and rapid screening and diagnosis for MDR-TB,which could cover the shortage of conventional DST,but genechip is strict with testing environment and needs strict training for the operators.关键词
肺结核/基因芯片/异烟肼/利福平/比例法药敏试验Key words
pulmonary tuberculosis/genechip/isoniazid/rifampicin/drug susceptibility testing by proportion method分类
社会科学引用本文复制引用
陈林,沈静,朱大冕,王晓英..基因芯片技术在检测痰涂阳肺结核患者结核分枝杆菌耐药性的应用效果[J].检验医学与临床,2017,14(9):1220-1223,4.基金项目
中国卫生部与比尔及梅琳达·盖茨基金会结核病防治项目(2009-04-01). （2009-04-01）
