检验医学与临床2017，Vol.14Issue(9)：1260-1261,1265,3.DOI:10.3969/j.issn.1672-9455.2017.09.021
血清外泌体来源的miRNA-122对肝癌诊断的研究
Vsalue of serum exosomes miRNA-122 detection in diagnosis of primary hepatocellular carcinoma
张云 1庹敏 2刘胜武3
作者信息
- 1. 武汉大学基础医学院 430071
- 2. 湖北省恩施土家族苗族自治州中心医院临床检验中心 445000
- 3. 湖北省恩施土家族苗族自治州中心医院乳腺外科 445000
- 折叠
摘要
Abstract
Objective To study the expression of serum exosomes miRNA-122 in hepatocellular carcinoma (HCC) and to investigate clinical value in diagnosis of HCC.Methods Serum of exosomes from 82 patients with HCC and 79 healthy controls were isolated and purified by exosomes extraction kit.The exosomes marker of Hsp70,CD63 and Alix were analyzed by Western blot,and the exosomes were identified by transmission electron microscopy.Serum of miRNA-122 from 79 patients with HCC and 82 healthy controls were detected by real-time quantitative PCR (qRT-PCR).The diagnostic values of exosomes miRNA-122 were analyzed furtherly.Results The expression of Hsp70,CD63 and Alix could be detected by Western blot in the isolated exosomes.The characteristics of exosomes were identified by TEM.The levels of exosomes miRNA-122 in HCC patients[log10(0.62±0.89)] was significantly higher than that of the control group[log10(-0.78±0.77)](P<0.001).The sensitivity of serum exosomes miRNA-122 in diagnosing HCC was 81.93%,and the specificity was 82.05%,AUC was 0.89(95%CI:0.84-0.94).Conclusion The serum exosomes miRNA-122 could be used as diagnosis markers of HCC.关键词
外泌体/miRNA-122/原发性肝癌/早期诊断Key words
exosomes/miRNA-122/hepatocellular carcinoma/early diagnosis分类
社会科学引用本文复制引用
张云,庹敏,刘胜武..血清外泌体来源的miRNA-122对肝癌诊断的研究[J].检验医学与临床,2017,14(9):1260-1261,1265,3.
