摘要
Abstract
Objective To study the relationship between follistatin-like protein 1(FSTL1) and acute myocardial infarction(AMI) and its possible mechanism.Methods Eighty cases of AMI were enrolled as the experimental group(AMI group),and 80 inpatients with negative AMI were recruited as the control group.Venous blood was collected for detecting plasma FSTL1 expression by ELISA.The non-coding RNA(ncRNAs,such as miRNAs,lncRNAs,circRNAs) and other functions were predicted by bioinformatics,and the preliminary verification was performed by adopting qRT-PCR.Results There were no statistical differences in age,gender and basic disease history between the two groups(P>0.05);total cholesterol and low density lipoprotein in the AMI group were higher than those in the control group,the difference was statistically significant(P<0.05);plasma FSTL1 expression in the AMI group was significantly higher than that in the control group with statistical difference(P>0.05),the ROC curve analysis found that the area under curve(AUC) of FSTL1 curve was 0.98(95%CI:0.96-0.99,P<0.05);the bioinformatics research found that the FSTL1 find mainly was related with miR-200 family(hsa-miR-200b-3p,hsa-miR-200c-3p and hsa-miR-429),related with lncRNAs of CTD-2630F21.1,CTB-92J24.2,RP11-473I1.10,C11orf95 and XIST,and related with circRNAs of C2orf29-hsa-circ-000665,MYO9B-hsa-circ-001731 and SMAD2-hsa-circ-000030;GO,KEGG and PANTHER prediction was mainly related with the signaling pathway of MAPK,PI3K Akt,TGF beta,Oxidative stress response,Inflammation mediated by chemokine and cytokine and Apoptosis.Plasma hsa-miR-200b-3p expression was significantly lower than the control group with statistical difference(P<0.05),and was negatively correlated with FSTL1(Pearson correlation coefficient was-0.665 7,P<0.05).Conclusion FSTL1 may be associated with AMI onset,and can be used as a biomarker of myocardial injury,which may involve in inflammation,oxidative stress and cell apoptosis in AMI by hsa-miR-200b-3p mediated regulation.关键词
血清卵泡抑素样蛋白1/心肌梗死/miR-200家族/生物标志物/非编码RNAsKey words
follistatin-like protein 1/acute myocardial infarction/miR-200 family/biomarkers/NcRNAs分类
社会科学
