空间科学学报2017,Vol.37Issue(2):185-191,7.DOI:10.11728/cjss2017.02.185
航天器AIT中心微生物多样性分析
Microbial Diversity Analysis in Spaceflight AIT Center
摘要
Abstract
The assembly of spacecraft in clean room environment is essential for the prevention of forward contamination.In this study,both high-throughput sequencing and culture-based method are used to study the microbial community structure and diversity in the AIT (Assembly,Integration and Test) center of the spaceflight.The result of high-throughput sequencing analysis show that in the AIT center the dominant genus of airborne bacteria is Bacillus (78.47%±1.59%),and the dominant fungi is Tremellales (8.97%±0.93%).However,culture-based analysis indicates that the Staphylococcus is the dominant bacteria,and no fungal colony is observed in the AIT center.The microbial diversity is evaluated by chaol index,Simpson index and Shannon index,and the results show that the bacterial diversity level is higher than that of fungal in the AIT center.关键词
AIT中心/高通量测序技术/培养法/微生物多样性Key words
AIT center/High-throughput sequencing/Culture-based method/Microbial diversity分类
航空航天引用本文复制引用
袁俊霞,印红,赵彪,张兰涛,曲溪,徐侃彦..航天器AIT中心微生物多样性分析[J].空间科学学报,2017,37(2):185-191,7.基金项目
国家自然科学基金青年科学基金项目(31300162)和中国航天科技集团公司科技创新研发项目共同资助 (31300162)